Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles
Photodynamic therapy (PDT) holds great potential to overcome limitations associated with common colorectal cancer (CRC) treatment approaches. Targeted photosensitiser (PS) delivery systems using nanoparticles (NPs) with targeting moieties are continually being designed, which are aimed at enhancing...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2024.2357693 |
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| author | Nokuphila Winifred Nompumelelo Simelane Heidi Abrahamse |
| author_facet | Nokuphila Winifred Nompumelelo Simelane Heidi Abrahamse |
| author_sort | Nokuphila Winifred Nompumelelo Simelane |
| collection | DOAJ |
| description | Photodynamic therapy (PDT) holds great potential to overcome limitations associated with common colorectal cancer (CRC) treatment approaches. Targeted photosensitiser (PS) delivery systems using nanoparticles (NPs) with targeting moieties are continually being designed, which are aimed at enhancing PS efficacy in CRC PDT. However, the optimisation of targeted PS delivery systems in most, in vitro PDT studies has been conducted on two dimensional (2D) monolayers cell cultures. In our present study, we developed a nano PS delivery system for in vitro cultured human colorectal three-dimensional multicellular spheroids (3D MCTS). PEGylated gold nanoparticles (PEG-AuNPs) were prepared and attached to ZnPcS4PS and further functionalised with specific CRC targeting anti-Guanylate Cyclase monoclonal antibodies(mAb). The ZnPcS4-AuNP-Anti-GCC Ab (BNC) nanoconjugates were successfully synthesised and their photodynamic effect investigated following exposure to laser irradiation and demonstrated enhanced anticancer effects in Caco-2 cells cultivated as 3D MCTS spheroids. Our findings suggest that targeted BNC nanoconjugates can improve the efficacy of PDT and highlight the potential of 3D MCTS tumour model for evaluating of targeted PDT. |
| format | Article |
| id | doaj-art-4034b72ebdc8402d961696d5a1d4c266 |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-4034b72ebdc8402d961696d5a1d4c2662024-12-06T10:48:00ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152130932010.1080/21691401.2024.2357693Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticlesNokuphila Winifred Nompumelelo Simelane0Heidi Abrahamse1Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South AfricaPhotodynamic therapy (PDT) holds great potential to overcome limitations associated with common colorectal cancer (CRC) treatment approaches. Targeted photosensitiser (PS) delivery systems using nanoparticles (NPs) with targeting moieties are continually being designed, which are aimed at enhancing PS efficacy in CRC PDT. However, the optimisation of targeted PS delivery systems in most, in vitro PDT studies has been conducted on two dimensional (2D) monolayers cell cultures. In our present study, we developed a nano PS delivery system for in vitro cultured human colorectal three-dimensional multicellular spheroids (3D MCTS). PEGylated gold nanoparticles (PEG-AuNPs) were prepared and attached to ZnPcS4PS and further functionalised with specific CRC targeting anti-Guanylate Cyclase monoclonal antibodies(mAb). The ZnPcS4-AuNP-Anti-GCC Ab (BNC) nanoconjugates were successfully synthesised and their photodynamic effect investigated following exposure to laser irradiation and demonstrated enhanced anticancer effects in Caco-2 cells cultivated as 3D MCTS spheroids. Our findings suggest that targeted BNC nanoconjugates can improve the efficacy of PDT and highlight the potential of 3D MCTS tumour model for evaluating of targeted PDT.https://www.tandfonline.com/doi/10.1080/21691401.2024.2357693Colorectal cancerphotodynamic therapynanoparticlesphotosensitiserthree-dimensional (3D) culture models |
| spellingShingle | Nokuphila Winifred Nompumelelo Simelane Heidi Abrahamse Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles Artificial Cells, Nanomedicine, and Biotechnology Colorectal cancer photodynamic therapy nanoparticles photosensitiser three-dimensional (3D) culture models |
| title | Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| title_full | Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| title_fullStr | Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| title_full_unstemmed | Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| title_short | Actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| title_sort | actively targeted photodynamic therapy in multicellular colorectal cancer spheroids via functionalised gold nanoparticles |
| topic | Colorectal cancer photodynamic therapy nanoparticles photosensitiser three-dimensional (3D) culture models |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2024.2357693 |
| work_keys_str_mv | AT nokuphilawinifrednompumelelosimelane activelytargetedphotodynamictherapyinmulticellularcolorectalcancerspheroidsviafunctionalisedgoldnanoparticles AT heidiabrahamse activelytargetedphotodynamictherapyinmulticellularcolorectalcancerspheroidsviafunctionalisedgoldnanoparticles |