Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals

Background and aims: Interferon-induced transmembrane protein 3 (IFITM3) plays an important antiviral role in the adaptive and innate immune response, it prevents hemifusion of the viral membrane and host cellular membrane in a broad spectrum of enveloped viruses, e.g. influenza A, ebola, marburg or...

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Main Authors: Esraa Abdelaziz, Amany Nafeh, Ahmed Hasanein, Hytham Abdalla, Ragaa Rashwan
Format: Article
Language:English
Published: Zagazig University, Faculty of Medicine 2024-05-01
Series:Microbes and Infectious Diseases
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Online Access:https://mid.journals.ekb.eg/article_339065_b99da2ae192bbe788379398ee4b0c0e3.pdf
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author Esraa Abdelaziz
Amany Nafeh
Ahmed Hasanein
Hytham Abdalla
Ragaa Rashwan
author_facet Esraa Abdelaziz
Amany Nafeh
Ahmed Hasanein
Hytham Abdalla
Ragaa Rashwan
author_sort Esraa Abdelaziz
collection DOAJ
description Background and aims: Interferon-induced transmembrane protein 3 (IFITM3) plays an important antiviral role in the adaptive and innate immune response, it prevents hemifusion of the viral membrane and host cellular membrane in a broad spectrum of enveloped viruses, e.g. influenza A, ebola, marburg or SARS-CoV. This study aimed to evaluate correlation of IFITM3 gene polymorphisms (rs12252) and infection susceptibility, severity and mortality rate of COVID-19 patients. Patients and methods: The IFITM3 SNP (rs12252) polymorphism was genotyped by (RT-PCR) in 100 SARS-CoV-2-negative controls and 100 SARS-CoV-2- positive, who admitted to Chest, Assiut University and other quarantine Hospitals respectively in Assiut city, Egypt. Results: The minor allele frequency (rs12252 (G)) were significantly more frequent in the patients compared to controls after age and sex matching (p-value = 0.011, OR (95% CI) =2.44 (1.20-4.97). This allele (G) was significantly more common among patients who ICU admitted than non-ICU admitted (p -value = 0.001, OR (95% CI) =3.78 (1.64-8.75)). Also, was significantly more frequent in dead patients than cured patients (p -value = 0.005, OR (95% CI) =3.33 (1.37-8.10)). Conclusion: The present study has shown significant association between G (the mutant type) allele variant of IFITM3 rs12252 and COVID-19 infection susceptibility and disease severity and mortality.
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publishDate 2024-05-01
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record_format Article
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spelling doaj-art-3f08f28fd7ae4132a08a4e074d4fa3492024-12-21T21:02:47ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-05-015245146210.21608/mid.2024.258008.1732339065Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University HospitalsEsraa Abdelaziz0Amany Nafeh1Ahmed Hasanein2Hytham Abdalla3Ragaa Rashwan4Microbiology and Immunology Department, Faculty of Pharmacy, Assiut University, Assiut- Egypt.Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, EgyptDepartment of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, EgyptDepartment of Chest Diseases, Faculty of Medicine, Al-Azhar University Assiut, Assiut, Egypt.Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, EgyptBackground and aims: Interferon-induced transmembrane protein 3 (IFITM3) plays an important antiviral role in the adaptive and innate immune response, it prevents hemifusion of the viral membrane and host cellular membrane in a broad spectrum of enveloped viruses, e.g. influenza A, ebola, marburg or SARS-CoV. This study aimed to evaluate correlation of IFITM3 gene polymorphisms (rs12252) and infection susceptibility, severity and mortality rate of COVID-19 patients. Patients and methods: The IFITM3 SNP (rs12252) polymorphism was genotyped by (RT-PCR) in 100 SARS-CoV-2-negative controls and 100 SARS-CoV-2- positive, who admitted to Chest, Assiut University and other quarantine Hospitals respectively in Assiut city, Egypt. Results: The minor allele frequency (rs12252 (G)) were significantly more frequent in the patients compared to controls after age and sex matching (p-value = 0.011, OR (95% CI) =2.44 (1.20-4.97). This allele (G) was significantly more common among patients who ICU admitted than non-ICU admitted (p -value = 0.001, OR (95% CI) =3.78 (1.64-8.75)). Also, was significantly more frequent in dead patients than cured patients (p -value = 0.005, OR (95% CI) =3.33 (1.37-8.10)). Conclusion: The present study has shown significant association between G (the mutant type) allele variant of IFITM3 rs12252 and COVID-19 infection susceptibility and disease severity and mortality.https://mid.journals.ekb.eg/article_339065_b99da2ae192bbe788379398ee4b0c0e3.pdfkeywords: sars-cov-2snpgenotypingrs12252rt-pcr
spellingShingle Esraa Abdelaziz
Amany Nafeh
Ahmed Hasanein
Hytham Abdalla
Ragaa Rashwan
Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
Microbes and Infectious Diseases
keywords: sars-cov-2
snp
genotyping
rs12252
rt-pcr
title Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
title_full Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
title_fullStr Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
title_full_unstemmed Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
title_short Interferon-induced transmembrane protein 3 (IFITM3) gene polymorphisms in COVID-19 patients in Assiut University Hospitals
title_sort interferon induced transmembrane protein 3 ifitm3 gene polymorphisms in covid 19 patients in assiut university hospitals
topic keywords: sars-cov-2
snp
genotyping
rs12252
rt-pcr
url https://mid.journals.ekb.eg/article_339065_b99da2ae192bbe788379398ee4b0c0e3.pdf
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