Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor
Vasoactive intestinal polypeptide (VIP) is a 28-amino acid neuropeptide with a multitude of diverse physiological functions, including vasodilation, immune modulation, and most notable gastrointestinal regulation. At present, VIP is determined using biochemical assays, which are time-consuming and r...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
|
| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000949 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849240137576218624 |
|---|---|
| author | Khalil K. Hussain Chloe Miller Mark Yeoman Bhavik Anil Patel |
| author_facet | Khalil K. Hussain Chloe Miller Mark Yeoman Bhavik Anil Patel |
| author_sort | Khalil K. Hussain |
| collection | DOAJ |
| description | Vasoactive intestinal polypeptide (VIP) is a 28-amino acid neuropeptide with a multitude of diverse physiological functions, including vasodilation, immune modulation, and most notable gastrointestinal regulation. At present, VIP is determined using biochemical assays, which are time-consuming and require complex multi-step sample preparation. Therefore, we focused on the development of the first electrochemical immunosensor for the detection of VIP in faecal pellets. Our VIP immunosensor was made by covalent immobilization of an anti-VIP antibody onto a thiophene-2-carboxylic acid conductive polymer layer, after deposition of gold nanostars onto a carbon black/polylactic acid 3D printed electrode. Scanning electron microscopy and electrochemical measurements confirmed layer-by-layer modification of the electrode to craft the VIP immunosensor. The immunosensor exhibited a linear range between 10 and 100 pM, with a limit of detection of 4.3 pM. The immunosensor was selective against electroactive compounds known to be present within the intestinal tract. We observed a reduction in VIP levels in faecal pellets from 24-month-old mice when compared to 12-month-old mice. This novel VIP electrochemical immunosensor can be an effective tool for diagnosis or prognosis of various diseases given the diverse physiological function of VIP. |
| format | Article |
| id | doaj-art-3ecf4b5544cc4b66a176b31f0f750f05 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-3ecf4b5544cc4b66a176b31f0f750f052025-08-20T04:00:43ZengElsevierBiosensors and Bioelectronics: X2590-13702025-10-012610066710.1016/j.biosx.2025.100667Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensorKhalil K. Hussain0Chloe Miller1Mark Yeoman2Bhavik Anil Patel3Centre for Lifelong Health, University of Brighton, Brighton, UK; School of Applied Sciences, University of Brighton, Brighton, UKCentre for Lifelong Health, University of Brighton, Brighton, UK; School of Applied Sciences, University of Brighton, Brighton, UKCentre for Lifelong Health, University of Brighton, Brighton, UK; School of Applied Sciences, University of Brighton, Brighton, UKCentre for Lifelong Health, University of Brighton, Brighton, UK; School of Applied Sciences, University of Brighton, Brighton, UK; Corresponding author. Centre for Lifelong Health, University of Brighton, Brighton, UK.Vasoactive intestinal polypeptide (VIP) is a 28-amino acid neuropeptide with a multitude of diverse physiological functions, including vasodilation, immune modulation, and most notable gastrointestinal regulation. At present, VIP is determined using biochemical assays, which are time-consuming and require complex multi-step sample preparation. Therefore, we focused on the development of the first electrochemical immunosensor for the detection of VIP in faecal pellets. Our VIP immunosensor was made by covalent immobilization of an anti-VIP antibody onto a thiophene-2-carboxylic acid conductive polymer layer, after deposition of gold nanostars onto a carbon black/polylactic acid 3D printed electrode. Scanning electron microscopy and electrochemical measurements confirmed layer-by-layer modification of the electrode to craft the VIP immunosensor. The immunosensor exhibited a linear range between 10 and 100 pM, with a limit of detection of 4.3 pM. The immunosensor was selective against electroactive compounds known to be present within the intestinal tract. We observed a reduction in VIP levels in faecal pellets from 24-month-old mice when compared to 12-month-old mice. This novel VIP electrochemical immunosensor can be an effective tool for diagnosis or prognosis of various diseases given the diverse physiological function of VIP.http://www.sciencedirect.com/science/article/pii/S2590137025000949ImmunosensorImpedanceVasoactive intestinal polypeptideVIPAgeingColon |
| spellingShingle | Khalil K. Hussain Chloe Miller Mark Yeoman Bhavik Anil Patel Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor Biosensors and Bioelectronics: X Immunosensor Impedance Vasoactive intestinal polypeptide VIP Ageing Colon |
| title | Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor |
| title_full | Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor |
| title_fullStr | Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor |
| title_full_unstemmed | Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor |
| title_short | Detecting age-related changes in faecal vasoactive intestinal polypeptide using a 3D-printed electrochemical immunosensor |
| title_sort | detecting age related changes in faecal vasoactive intestinal polypeptide using a 3d printed electrochemical immunosensor |
| topic | Immunosensor Impedance Vasoactive intestinal polypeptide VIP Ageing Colon |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000949 |
| work_keys_str_mv | AT khalilkhussain detectingagerelatedchangesinfaecalvasoactiveintestinalpolypeptideusinga3dprintedelectrochemicalimmunosensor AT chloemiller detectingagerelatedchangesinfaecalvasoactiveintestinalpolypeptideusinga3dprintedelectrochemicalimmunosensor AT markyeoman detectingagerelatedchangesinfaecalvasoactiveintestinalpolypeptideusinga3dprintedelectrochemicalimmunosensor AT bhavikanilpatel detectingagerelatedchangesinfaecalvasoactiveintestinalpolypeptideusinga3dprintedelectrochemicalimmunosensor |