Enhanced Genome Editing Activity with Novel Chimeric ScCas9 Variants in Rice

Abstract The Streptococcus canis Cas9 protein (ScCas9) recognizes the NNG protospacer adjacent motif (PAM), offering a wider range of targets than that offered by the commonly used S. pyogenes Cas9 protein (SpCas9). However, both ScCas9 and its evolved Sc++ variant still exhibit low genome editing e...

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Bibliographic Details
Main Authors: Zhen Liang, Yuqing Wu, Shuke Deng, Sha Wei, Kai Zhang, Yingjie Guo
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202411549
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