Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model
Purpose To evaluate the safety and efficacy of performing histotripsy through overlying gas-filled bowel in an ex vivo swine model.Methods An ex vivo model was created to simulate histotripsy treatment of solid organs through gas-filled bowel. Spherical 2.5 cm histotripsy treatments were performed i...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | International Journal of Hyperthermia |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2024.2369305 |
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| author | Meridith A. Kisting James K. White Sarvesh Periyasamy Ayca Z. Kutlu Adrienne L. Kisting Xiaofei Zhang Lu Mao Paul F. Laeseke Martin G. Wagner Eli Vlaisavljevich Fred T. Lee Timothy J. Ziemlewicz |
| author_facet | Meridith A. Kisting James K. White Sarvesh Periyasamy Ayca Z. Kutlu Adrienne L. Kisting Xiaofei Zhang Lu Mao Paul F. Laeseke Martin G. Wagner Eli Vlaisavljevich Fred T. Lee Timothy J. Ziemlewicz |
| author_sort | Meridith A. Kisting |
| collection | DOAJ |
| description | Purpose To evaluate the safety and efficacy of performing histotripsy through overlying gas-filled bowel in an ex vivo swine model.Methods An ex vivo model was created to simulate histotripsy treatment of solid organs through gas-filled bowel. Spherical 2.5 cm histotripsy treatments were performed in agar phantoms for each of five treatment groups: 1) control with no overlying bowel (n = 6), 2) bowel 0 cm above phantom (n = 6), 3) bowel 1 cm above phantom (n = 6), 4) bowel 2 cm above phantom (n = 6), and 5) bowel 0 cm above the phantom with increased treatment amplitude (n = 6). Bowel was inspected for gross and microscopic damage, and treatment zones were measured. A ray-tracing simulation estimated the percentage of therapeutic beam path blockage by bowel in each scenario.Results All histotripsy treatments through partial blockage were successful (24/24). No visible or microscopic damage was observed to intervening bowel. Partial blockage resulted in a small increase in treatment volume compared to controls (p = 0.002 and p = 0.036 for groups with bowel 0 cm above the phantom, p > 0.3 for bowel 1 cm and 2 cm above the phantom). Gas-filled bowel was estimated to have blocked 49.6%, 35.0%, and 27.3% of the therapeutic beam at 0, 1, and 2 cm, respectively.Conclusion Histotripsy has the potential to be applied through partial gas blockage of the therapeutic beam path, as shown by this ex vivo small bowel model. Further work in an in vivo survival model appears indicated. |
| format | Article |
| id | doaj-art-3e7634bae5844f729cd6e1a064be5cf6 |
| institution | Kabale University |
| issn | 0265-6736 1464-5157 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj-art-3e7634bae5844f729cd6e1a064be5cf62025-01-03T09:30:27ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572024-12-0141110.1080/02656736.2024.2369305Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine modelMeridith A. Kisting0James K. White1Sarvesh Periyasamy2Ayca Z. Kutlu3Adrienne L. Kisting4Xiaofei Zhang5Lu Mao6Paul F. Laeseke7Martin G. Wagner8Eli Vlaisavljevich9Fred T. Lee10Timothy J. Ziemlewicz11Departments of Radiology, University of Wisconsin, Madison, WI, USADepartments of Radiology, University of Wisconsin, Madison, WI, USADepartment of Radiology, Stanford University, Stanford, CA, USADepartments of Radiology, University of Wisconsin, Madison, WI, USADepartments of Radiology, University of Wisconsin, Madison, WI, USASchool of Medicine Department of Pathology, NY University, Long Island, NY, USADepartments of Biostatistics, University of Wisconsin, Madison, WI, USADepartments of Radiology, University of Wisconsin, Madison, WI, USADepartments of Radiology, University of Wisconsin, Madison, WI, USADepartment of Biomedical Engineering, VA Tech, Blacksburg, VA, USADepartments of Radiology, University of Wisconsin, Madison, WI, USADepartments of Radiology, University of Wisconsin, Madison, WI, USAPurpose To evaluate the safety and efficacy of performing histotripsy through overlying gas-filled bowel in an ex vivo swine model.Methods An ex vivo model was created to simulate histotripsy treatment of solid organs through gas-filled bowel. Spherical 2.5 cm histotripsy treatments were performed in agar phantoms for each of five treatment groups: 1) control with no overlying bowel (n = 6), 2) bowel 0 cm above phantom (n = 6), 3) bowel 1 cm above phantom (n = 6), 4) bowel 2 cm above phantom (n = 6), and 5) bowel 0 cm above the phantom with increased treatment amplitude (n = 6). Bowel was inspected for gross and microscopic damage, and treatment zones were measured. A ray-tracing simulation estimated the percentage of therapeutic beam path blockage by bowel in each scenario.Results All histotripsy treatments through partial blockage were successful (24/24). No visible or microscopic damage was observed to intervening bowel. Partial blockage resulted in a small increase in treatment volume compared to controls (p = 0.002 and p = 0.036 for groups with bowel 0 cm above the phantom, p > 0.3 for bowel 1 cm and 2 cm above the phantom). Gas-filled bowel was estimated to have blocked 49.6%, 35.0%, and 27.3% of the therapeutic beam at 0, 1, and 2 cm, respectively.Conclusion Histotripsy has the potential to be applied through partial gas blockage of the therapeutic beam path, as shown by this ex vivo small bowel model. Further work in an in vivo survival model appears indicated.https://www.tandfonline.com/doi/10.1080/02656736.2024.2369305Histotripsyfocused ultrasoundex vivo modelsmall bowelgas blockage |
| spellingShingle | Meridith A. Kisting James K. White Sarvesh Periyasamy Ayca Z. Kutlu Adrienne L. Kisting Xiaofei Zhang Lu Mao Paul F. Laeseke Martin G. Wagner Eli Vlaisavljevich Fred T. Lee Timothy J. Ziemlewicz Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model International Journal of Hyperthermia Histotripsy focused ultrasound ex vivo model small bowel gas blockage |
| title | Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model |
| title_full | Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model |
| title_fullStr | Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model |
| title_full_unstemmed | Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model |
| title_short | Safety and efficacy of histotripsy delivery through overlying gas-filled small bowel in an ex vivo swine model |
| title_sort | safety and efficacy of histotripsy delivery through overlying gas filled small bowel in an ex vivo swine model |
| topic | Histotripsy focused ultrasound ex vivo model small bowel gas blockage |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2024.2369305 |
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