Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats
ABSTRACT Purpose: We aimed to investigate the role of gallic acid treatment on spinal cord tissues after spinal cord injury (SCI) and its relationship with endoplasmic reticulum (ER) stress by histochemical, immunohistochemical, and in-silico techniques. Methods: Thirty female Wistar albino rats w...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-01-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100205&lng=en&tlng=en |
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| author | Abdulmutalip Karaaslanlı Mehmet Cudi Tuncer Fırat Aşır Tuğcan Korak |
| author_facet | Abdulmutalip Karaaslanlı Mehmet Cudi Tuncer Fırat Aşır Tuğcan Korak |
| author_sort | Abdulmutalip Karaaslanlı |
| collection | DOAJ |
| description | ABSTRACT Purpose: We aimed to investigate the role of gallic acid treatment on spinal cord tissues after spinal cord injury (SCI) and its relationship with endoplasmic reticulum (ER) stress by histochemical, immunohistochemical, and in-silico techniques. Methods: Thirty female Wistar albino rats were divided into three groups: sham, SCI, and SCI+gallic acid. SCI was induced by dropping a 15-g weight onto the exposed T10-T11 spinal cord segment. The SCI+gallic acid group received 25 mg/kg of gallic acid intraperitoneally daily for one week. Histopathological, immunohistochemical, and silico analyses were performed. Results: Histological analysis revealed improved neural cell survival and tissue integrity in the SCI+gallic acid group compared to the SCI group. Caspase-12 expression was significantly increased in the SCI group, indicating elevated ER stress and apoptosis. Gallic acid treatment resulted in a marked reduction in caspase-12 expression in neurons, neuroglia, and endothelial cells, suggesting decreased ER stress. Conclusion: Gallic acid exhibits significant neuroprotective effects against ER stress and cellular damage in a rat model of SCI. The in-silico analysis revealed apoptotic and immune-related pathways in which gallic acid showed neuroprotective effects by regulating caspase-12. These results suggest that gallic acid may be a promising therapeutic agent for mitigating secondary damage post-SCI. |
| format | Article |
| id | doaj-art-3b4c77e329de4b80a01e600b71d84eb7 |
| institution | Kabale University |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-3b4c77e329de4b80a01e600b71d84eb72025-01-14T07:41:08ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-01-014010.1590/acb400925Gallic acid showed neuroprotection against endoplasmic reticulum stress in ratsAbdulmutalip Karaaslanlıhttps://orcid.org/0009-0005-1046-3484Mehmet Cudi Tuncerhttps://orcid.org/0000-0001-7317-5467Fırat Aşırhttps://orcid.org/0000-0002-6384-9146Tuğcan Korakhttps://orcid.org/0000-0003-4902-4022ABSTRACT Purpose: We aimed to investigate the role of gallic acid treatment on spinal cord tissues after spinal cord injury (SCI) and its relationship with endoplasmic reticulum (ER) stress by histochemical, immunohistochemical, and in-silico techniques. Methods: Thirty female Wistar albino rats were divided into three groups: sham, SCI, and SCI+gallic acid. SCI was induced by dropping a 15-g weight onto the exposed T10-T11 spinal cord segment. The SCI+gallic acid group received 25 mg/kg of gallic acid intraperitoneally daily for one week. Histopathological, immunohistochemical, and silico analyses were performed. Results: Histological analysis revealed improved neural cell survival and tissue integrity in the SCI+gallic acid group compared to the SCI group. Caspase-12 expression was significantly increased in the SCI group, indicating elevated ER stress and apoptosis. Gallic acid treatment resulted in a marked reduction in caspase-12 expression in neurons, neuroglia, and endothelial cells, suggesting decreased ER stress. Conclusion: Gallic acid exhibits significant neuroprotective effects against ER stress and cellular damage in a rat model of SCI. The in-silico analysis revealed apoptotic and immune-related pathways in which gallic acid showed neuroprotective effects by regulating caspase-12. These results suggest that gallic acid may be a promising therapeutic agent for mitigating secondary damage post-SCI.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100205&lng=en&tlng=enGallic AcidCaspasesEndoplasmic Reticulum StressSpinal Cord Injuries |
| spellingShingle | Abdulmutalip Karaaslanlı Mehmet Cudi Tuncer Fırat Aşır Tuğcan Korak Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats Acta Cirúrgica Brasileira Gallic Acid Caspases Endoplasmic Reticulum Stress Spinal Cord Injuries |
| title | Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| title_full | Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| title_fullStr | Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| title_full_unstemmed | Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| title_short | Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| title_sort | gallic acid showed neuroprotection against endoplasmic reticulum stress in rats |
| topic | Gallic Acid Caspases Endoplasmic Reticulum Stress Spinal Cord Injuries |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100205&lng=en&tlng=en |
| work_keys_str_mv | AT abdulmutalipkaraaslanlı gallicacidshowedneuroprotectionagainstendoplasmicreticulumstressinrats AT mehmetcudituncer gallicacidshowedneuroprotectionagainstendoplasmicreticulumstressinrats AT fıratasır gallicacidshowedneuroprotectionagainstendoplasmicreticulumstressinrats AT tugcankorak gallicacidshowedneuroprotectionagainstendoplasmicreticulumstressinrats |