Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid
The goal of the current research was to establisha quick and practical fluorimetric technique for fingolimod analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high-fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sul...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Green Analytical Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772577424000818 |
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| author | Hesham Salem Dina Z. Mazen Habeba Ahmed joy Fares Amany Abdelaziz |
| author_facet | Hesham Salem Dina Z. Mazen Habeba Ahmed joy Fares Amany Abdelaziz |
| author_sort | Hesham Salem |
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| description | The goal of the current research was to establisha quick and practical fluorimetric technique for fingolimod analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high-fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 325 nm following excitation at 475 nm. With a correlation coefficient of 0.9999, the association between emission intensity and fingolimod concentration was linear between 5 and 150 ng mL-1. The quantitation limit was 2.059 ng mL-1,while the detection limit was 0.679 ng mL-1. The buffer type, pH and concentration, type of surfactant and concentration, and finally the diluting solvent were among the reaction conditions that were closely examined. With great precision and reliability, the drug in question was quantified using this method in both spiked human plasma and capsule formulations. The proposed method's level of greenness was assessed using two methodologies: the analytical greenness metric (AGREE) and the Green Analytical Procedure Index (GAPI). |
| format | Article |
| id | doaj-art-3a845161fd8a45dfb0d43f1bf20787c1 |
| institution | Kabale University |
| issn | 2772-5774 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Green Analytical Chemistry |
| spelling | doaj-art-3a845161fd8a45dfb0d43f1bf20787c12024-12-08T06:13:25ZengElsevierGreen Analytical Chemistry2772-57742024-12-0111100172Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluidHesham Salem0Dina Z. Mazen1Habeba Ahmed2joy Fares3Amany Abdelaziz4Corresponding author.; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, EgyptThe goal of the current research was to establisha quick and practical fluorimetric technique for fingolimod analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high-fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 325 nm following excitation at 475 nm. With a correlation coefficient of 0.9999, the association between emission intensity and fingolimod concentration was linear between 5 and 150 ng mL-1. The quantitation limit was 2.059 ng mL-1,while the detection limit was 0.679 ng mL-1. The buffer type, pH and concentration, type of surfactant and concentration, and finally the diluting solvent were among the reaction conditions that were closely examined. With great precision and reliability, the drug in question was quantified using this method in both spiked human plasma and capsule formulations. The proposed method's level of greenness was assessed using two methodologies: the analytical greenness metric (AGREE) and the Green Analytical Procedure Index (GAPI).http://www.sciencedirect.com/science/article/pii/S2772577424000818SpectrofluorimetryFingolimodZincSodium dodecyl sulfate |
| spellingShingle | Hesham Salem Dina Z. Mazen Habeba Ahmed joy Fares Amany Abdelaziz Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid Green Analytical Chemistry Spectrofluorimetry Fingolimod Zinc Sodium dodecyl sulfate |
| title | Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid |
| title_full | Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid |
| title_fullStr | Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid |
| title_full_unstemmed | Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid |
| title_short | Utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation; Application to dosage and spiked biological fluid |
| title_sort | utility of green chemistry for spectrofluorometric determination of fingolimod via metal complexation application to dosage and spiked biological fluid |
| topic | Spectrofluorimetry Fingolimod Zinc Sodium dodecyl sulfate |
| url | http://www.sciencedirect.com/science/article/pii/S2772577424000818 |
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