STATISTICAL OPTIMIZATION OF CULTIVATION CONDITIONS FOR SACCHAROMYCES BOULARDII VIA CENTRAL COMPOSITE DESIGN
Saccharomyces boulardii (S. boulardii) is used in many countries as both a preventive and therapeutic agent for diarrhea and other gastrointestinal disorders caused by the administration of antimicrobial agents. However, there are few reports on its culture conditions. Therefore, the aim of this...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2020-06-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5107 |
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| Summary: | Saccharomyces boulardii (S. boulardii) is used in many
countries as both a preventive and therapeutic agent for diarrhea and other
gastrointestinal disorders caused by the administration of antimicrobial agents.
However, there are few reports on its culture conditions. Therefore, the aim of
this study was to optimize the cultivation conditions of S. boulardii by single
factor experiment and central composite design of Response Surface
Methodology (RSM). The effects of temperature (28, 31, 34, 37, 41 °C), initial
pH value (2, 4, 6, 8 and 10), liquid loading (25, 50, 75, 100 and 125 mL in 250
mL triangular flask), inoculum size (1, 3, 5, 7 and 9 %), and shaking speed
(140, 160, 180, 200, 220 rot∙min-1) were investigated. Among these variables,
the combination of 37 °C temperature, 6.4 the initial pH value, 250 mL
triangular flask with 52 mL medium volume, 3 % inoculum size, and 181
rot∙min-1 shaking speed were found to be the most suitable cultivation
conditions. Under the optimized conditions, the maximal yeast count was
(1.81±0.06)×108
CFU∙mL-1, which was in a good agreement with the predicted
value of the model. This study has provided useful information on how to
improve the viable cells of yeast cells for S. boulardii culture. |
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| ISSN: | 1582-540X 1582-540X |