Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution
IntroductionMyeloid cells trafficking from the periphery to the central nervous system are key players in multiple sclerosis (MS) through antigen presentation, cytokine secretion and repair processes.MethodsCombination of mass cytometry on blood cells from 60 MS patients at diagnosis and 29 healthy...
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2025-01-01
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| author | Stéphane Rodriguez Stéphane Rodriguez Laura Couloume Juliette Ferrant Juliette Ferrant Nicolas Vince Marion Mandon Marion Mandon Rachel Jean Rachel Jean Celine Monvoisin Simon Leonard Simon Le Gallou Simon Le Gallou Nayane S. B. Silva Nayane S. B. Silva Sonia Bourguiba-Hachemi David Laplaud David Laplaud Alexandra Garcia Romain Casey Romain Casey Romain Casey Romain Casey Helene Zephir Anne Kerbrat Gilles Edan Emmanuelle Lepage Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Guillaume Mathey Guillaume Mathey Pierre-Antoine Gourraud Pierre-Antoine Gourraud Karin Tarte Karin Tarte Celine Delaloy Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel |
| author_facet | Stéphane Rodriguez Stéphane Rodriguez Laura Couloume Juliette Ferrant Juliette Ferrant Nicolas Vince Marion Mandon Marion Mandon Rachel Jean Rachel Jean Celine Monvoisin Simon Leonard Simon Le Gallou Simon Le Gallou Nayane S. B. Silva Nayane S. B. Silva Sonia Bourguiba-Hachemi David Laplaud David Laplaud Alexandra Garcia Romain Casey Romain Casey Romain Casey Romain Casey Helene Zephir Anne Kerbrat Gilles Edan Emmanuelle Lepage Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Guillaume Mathey Guillaume Mathey Pierre-Antoine Gourraud Pierre-Antoine Gourraud Karin Tarte Karin Tarte Celine Delaloy Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel |
| author_sort | Stéphane Rodriguez |
| collection | DOAJ |
| description | IntroductionMyeloid cells trafficking from the periphery to the central nervous system are key players in multiple sclerosis (MS) through antigen presentation, cytokine secretion and repair processes.MethodsCombination of mass cytometry on blood cells from 60 MS patients at diagnosis and 29 healthy controls, along with single cell RNA sequencing on paired blood and cerebrospinal fluid (CSF) samples from 5 MS patients were used for myeloid cells detailing.ResultsMyeloid compartment study demonstrated an enrichment of a peculiar classical monocyte population in 22% of MS patients at the time of diagnosis. Notably, this patients’ subgroup exhibited a more aggressive disease phenotype two years post-diagnosis. This monocytic population, detected in both the CSF and blood, was characterized by CD206, CD209, CCR5 and CCR2 expression, and was found to be more frequent in MS patients carrying the HLA-DRB1*15:01 allele. Furthermore, pathways analysis predicted that these cells had antigen presentation capabilities coupled with pro-inflammatory phenotype.DiscussionAltogether, these results point toward the amplification of a specific and pathogenic myeloid cell subset in MS patients with genetic susceptibilities. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-39dcf655253348839790e0e5abb3f93b2025-01-08T08:14:48ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.14948421494842Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolutionStéphane Rodriguez0Stéphane Rodriguez1Laura Couloume2Juliette Ferrant3Juliette Ferrant4Nicolas Vince5Marion Mandon6Marion Mandon7Rachel Jean8Rachel Jean9Celine Monvoisin10Simon Leonard11Simon Le Gallou12Simon Le Gallou13Nayane S. B. Silva14Nayane S. B. Silva15Sonia Bourguiba-Hachemi16David Laplaud17David Laplaud18Alexandra Garcia19Romain Casey20Romain Casey21Romain Casey22Romain Casey23Helene Zephir24Anne Kerbrat25Gilles Edan26Emmanuelle Lepage27Eric Thouvenot28Eric Thouvenot29Aurelie Ruet30Aurelie Ruet31Guillaume Mathey32Guillaume Mathey33Pierre-Antoine Gourraud34Pierre-Antoine Gourraud35Karin Tarte36Karin Tarte37Celine Delaloy38Patricia Amé39Patricia Amé40Mikael Roussel41Mikael Roussel42Laure Michel43Laure Michel44Laure Michel45Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceSão Paulo State University, Molecular Genetics and Bioinformatics Laboratory, School of Medicine, Botucatu, BrazilInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceService de Neurologie, Centre Hospitalier Universitaire (CHU) Nantes, CRC-SEP Pays de la Loire, CIC 1413, Nantes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceLyon University, University Claude Bernard Lyon 1, Lyon, FranceHospices Civils de Lyon, Neurology Department, Sclérose en Plaques, Pathologies de la Myéline et Neuro-Inflammation, Bron, FranceObservatoire Français de la Sclérose en Plaques, Centre de Recherche en Neurosciences de Lyon, INSERM 1028 and CNRS UMR 5292, Lyon, FranceEUGENE DEVIC EDMUS Foundation against Multiple Sclerosis, State-Approved Foundation, Bron, France0Lille University, Inserm U1172, Lille University Hospital, Lille, France1Neurology Department, Rennes Clinical Investigation Centre, Rennes University Hospital-Rennes University-Institut National de la Santé et de la Recherche Médicale (INSERM), Rennes, France1Neurology Department, Rennes Clinical Investigation Centre, Rennes University Hospital-Rennes University-Institut National de la Santé et de la Recherche Médicale (INSERM), Rennes, France1Neurology Department, Rennes Clinical Investigation Centre, Rennes University Hospital-Rennes University-Institut National de la Santé et de la Recherche Médicale (INSERM), Rennes, France2Department of Neurology, Nimes University Hospital, Nimes, France3Institut de Génomique Fonctionnelle, UMR5203, Inserm 1191, Université de Montpellier, Montpellier, France4Neurocentre Magendie, Institut National de la Santé et de la Recherche Médicale (INSERM) U1215, Bordeaux, France5CHU de Bordeaux, Department of Neurology, Bordeaux, France6Department of Neurology, Nancy University Hospital, Nancy, France7Université de Lorraine, Inserm, INSPIIRE, Nancy, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire (CHU) Nantes, Nantes University, Center for Research in Transplantation and Translational Immunology, UMR 1064, Nantes, FranceService de Neurologie, Centre Hospitalier Universitaire (CHU) Nantes, CRC-SEP Pays de la Loire, CIC 1413, Nantes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceInstitut National de la Santé et de la Recherche Médicale (INSERM), Unité Mixte de Recherche U1236, Université Rennes, Etablissement Français du Sang Bretagne, LabEx IGO, Rennes, FrancePole Biologie-Centre Hospitalier Universitaire (CHU) Rennes, Rennes, FranceObservatoire Français de la Sclérose en Plaques, Centre de Recherche en Neurosciences de Lyon, INSERM 1028 and CNRS UMR 5292, Lyon, FranceIntroductionMyeloid cells trafficking from the periphery to the central nervous system are key players in multiple sclerosis (MS) through antigen presentation, cytokine secretion and repair processes.MethodsCombination of mass cytometry on blood cells from 60 MS patients at diagnosis and 29 healthy controls, along with single cell RNA sequencing on paired blood and cerebrospinal fluid (CSF) samples from 5 MS patients were used for myeloid cells detailing.ResultsMyeloid compartment study demonstrated an enrichment of a peculiar classical monocyte population in 22% of MS patients at the time of diagnosis. Notably, this patients’ subgroup exhibited a more aggressive disease phenotype two years post-diagnosis. This monocytic population, detected in both the CSF and blood, was characterized by CD206, CD209, CCR5 and CCR2 expression, and was found to be more frequent in MS patients carrying the HLA-DRB1*15:01 allele. Furthermore, pathways analysis predicted that these cells had antigen presentation capabilities coupled with pro-inflammatory phenotype.DiscussionAltogether, these results point toward the amplification of a specific and pathogenic myeloid cell subset in MS patients with genetic susceptibilities.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1494842/fullmultiple sclerosiscerebrospinal fluidclassical monocytedisabilityantigen presentation |
| spellingShingle | Stéphane Rodriguez Stéphane Rodriguez Laura Couloume Juliette Ferrant Juliette Ferrant Nicolas Vince Marion Mandon Marion Mandon Rachel Jean Rachel Jean Celine Monvoisin Simon Leonard Simon Le Gallou Simon Le Gallou Nayane S. B. Silva Nayane S. B. Silva Sonia Bourguiba-Hachemi David Laplaud David Laplaud Alexandra Garcia Romain Casey Romain Casey Romain Casey Romain Casey Helene Zephir Anne Kerbrat Gilles Edan Emmanuelle Lepage Eric Thouvenot Eric Thouvenot Aurelie Ruet Aurelie Ruet Guillaume Mathey Guillaume Mathey Pierre-Antoine Gourraud Pierre-Antoine Gourraud Karin Tarte Karin Tarte Celine Delaloy Patricia Amé Patricia Amé Mikael Roussel Mikael Roussel Laure Michel Laure Michel Laure Michel Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution Frontiers in Immunology multiple sclerosis cerebrospinal fluid classical monocyte disability antigen presentation |
| title | Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| title_full | Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| title_fullStr | Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| title_full_unstemmed | Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| title_short | Blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| title_sort | blood immunophenotyping of multiple sclerosis patients at diagnosis identifies a classical monocyte subset associated to disease evolution |
| topic | multiple sclerosis cerebrospinal fluid classical monocyte disability antigen presentation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1494842/full |
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