Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae)
Abstract Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level geno...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-04360-3 |
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| author | Hao He Yongxin Zhao Dengyu Zheng Huiyun Kuang Fei Shen Kang He Haiyan Zhang Changjiang Zhao Lu Jiang Da Xiao Su Wang Zhenying Wang Shuai Zhan Jianhua Wei Xiaozeng Yang Zhongyi Wu Chun Zhang |
| author_facet | Hao He Yongxin Zhao Dengyu Zheng Huiyun Kuang Fei Shen Kang He Haiyan Zhang Changjiang Zhao Lu Jiang Da Xiao Su Wang Zhenying Wang Shuai Zhan Jianhua Wei Xiaozeng Yang Zhongyi Wu Chun Zhang |
| author_sort | Hao He |
| collection | DOAJ |
| description | Abstract Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level genome assembly of M. hieroglyphica, with contig N50 of 18.62 Mb and scaffold N50 of 241.916 Mb. Using 156x Hi-C data, we anchored a total length of 2,313 Mb (91.9% of the genome) to nine chromosomes. We predicted 46,561 genes using an integrated approach. The completeness of the final reported genome and proteome were assessed by BUSCO and show high values of 99.4% and 95.2%, respectively. The high-quality chromosome-level genome assembly of M. hieroglyphica will be a useful molecular resource for the communities of both beetle and crop protection. |
| format | Article |
| id | doaj-art-39d4a1cb1f2641c79e1269a40d8d5df4 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-39d4a1cb1f2641c79e1269a40d8d5df42025-01-05T12:08:20ZengNature PortfolioScientific Data2052-44632025-01-011211810.1038/s41597-024-04360-3Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae)Hao He0Yongxin Zhao1Dengyu Zheng2Huiyun Kuang3Fei Shen4Kang He5Haiyan Zhang6Changjiang Zhao7Lu Jiang8Da Xiao9Su Wang10Zhenying Wang11Shuai Zhan12Jianhua Wei13Xiaozeng Yang14Zhongyi Wu15Chun Zhang16Institute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesCrop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesState Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang UniversityCollege of Agriculture, Heilongjiang Bayi Agricultural UniversityCollege of Agriculture, Heilongjiang Bayi Agricultural UniversityKey Laboratory of Economic and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural UniversityInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry ScienceInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry ScienceInstitute of Plant Protection, Chinese Academy of Agricultural SciencesKey Laboratory of Plant Design, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesInstitute of Biotechnology, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing Academy of Agriculture and Forestry SciencesAbstract Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level genome assembly of M. hieroglyphica, with contig N50 of 18.62 Mb and scaffold N50 of 241.916 Mb. Using 156x Hi-C data, we anchored a total length of 2,313 Mb (91.9% of the genome) to nine chromosomes. We predicted 46,561 genes using an integrated approach. The completeness of the final reported genome and proteome were assessed by BUSCO and show high values of 99.4% and 95.2%, respectively. The high-quality chromosome-level genome assembly of M. hieroglyphica will be a useful molecular resource for the communities of both beetle and crop protection.https://doi.org/10.1038/s41597-024-04360-3 |
| spellingShingle | Hao He Yongxin Zhao Dengyu Zheng Huiyun Kuang Fei Shen Kang He Haiyan Zhang Changjiang Zhao Lu Jiang Da Xiao Su Wang Zhenying Wang Shuai Zhan Jianhua Wei Xiaozeng Yang Zhongyi Wu Chun Zhang Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) Scientific Data |
| title | Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) |
| title_full | Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) |
| title_fullStr | Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) |
| title_full_unstemmed | Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) |
| title_short | Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae) |
| title_sort | chromosome level genome assembly of monolepta hieroglyphica two spotted leaf beetle coleoptera chrysomelidae |
| url | https://doi.org/10.1038/s41597-024-04360-3 |
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