Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes
In this work, we present the detection of proteins expressed by poxvirus with fiber-optic probes based on a semi-distributed interferometer (SDI) assisted by a fiber Bragg grating (FBG), performing the measurement directly into a wastewater sample. Modern biosafety applications benefit from real-tim...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180424000813 |
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| author | Albina Abdossova Aina Adilzhankyzy Kuanysh Seitkamal Massimo Olivero Guido Perrone Wilfried Blanc Luca Vangelista Daniele Tosi |
| author_facet | Albina Abdossova Aina Adilzhankyzy Kuanysh Seitkamal Massimo Olivero Guido Perrone Wilfried Blanc Luca Vangelista Daniele Tosi |
| author_sort | Albina Abdossova |
| collection | DOAJ |
| description | In this work, we present the detection of proteins expressed by poxvirus with fiber-optic probes based on a semi-distributed interferometer (SDI) assisted by a fiber Bragg grating (FBG), performing the measurement directly into a wastewater sample. Modern biosafety applications benefit from real-time, dynamic-sensing technologies that can perform diagnostic tasks into a wide set of analytes, with a particular emphasis on wastewater, which appears to collect a significant number of viral titers in urban and indoor environments. The SDI/FBG probe can perform substantial progress in this field, as it embeds a dual sensitivity mechanism to refractive index changes (sensitivity up to 266.1 dB/RIU (refractive index units)) that can be exploited in biosensing, while simultaneously having the capability to measure the temperature (sensitivity 9.888 pm/°C), thus providing an intrinsic cross-sensitivity compensation. In addition, a standard FBG analyzer can be used as an interrogator, improving affordability and real-time detection over previous works. The probes have been functionalized with antibodies specific for L1, A27 and A33 vaccinia virus proteins, performing detection of a protein concentration in a scenario compatible with online viral threat detection. Direct detection of wastewater samples shows that the L1-functionalized sensor has a higher response, 9.1–11.3 times higher than A33 and A27, respectively, with a maximum response of up to 1.99 dB and excellent specificity. Dynamic detection in wastewater shows that the sensors have a response over multiple detection cycles, with a sensitivity of 0.024–0.153 dB for each 10-fold increase of concentration. |
| format | Article |
| id | doaj-art-37922fa2431f4a42b8e5328e6aa20c7e |
| institution | Kabale University |
| issn | 2214-1804 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-37922fa2431f4a42b8e5328e6aa20c7e2024-12-09T04:27:26ZengElsevierSensing and Bio-Sensing Research2214-18042024-12-0146100699Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probesAlbina Abdossova0Aina Adilzhankyzy1Kuanysh Seitkamal2Massimo Olivero3Guido Perrone4Wilfried Blanc5Luca Vangelista6Daniele Tosi7School of Engineering and Digital Sciences, Nazarbayev University, Astana, KazakhstanSchool of Engineering and Digital Sciences, Nazarbayev University, Astana, KazakhstanLaboratory of Biosensors and Bioinstruments, National Laboratory Astana, Astana, Kazakhstan; School of Medicine, Nazarbayev University, Astana, KazakhstanDepartment of Electronics and Telecommunications, Politecnico di Torino, Turin, ItalyDepartment of Electronics and Telecommunications, Politecnico di Torino, Turin, ItalyUniversité Côte d'Azur, INPHYNI, CNRS UMR7010, 06200 Nice, FranceDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalySchool of Engineering and Digital Sciences, Nazarbayev University, Astana, Kazakhstan; Laboratory of Biosensors and Bioinstruments, National Laboratory Astana, Astana, Kazakhstan; Corresponding author.In this work, we present the detection of proteins expressed by poxvirus with fiber-optic probes based on a semi-distributed interferometer (SDI) assisted by a fiber Bragg grating (FBG), performing the measurement directly into a wastewater sample. Modern biosafety applications benefit from real-time, dynamic-sensing technologies that can perform diagnostic tasks into a wide set of analytes, with a particular emphasis on wastewater, which appears to collect a significant number of viral titers in urban and indoor environments. The SDI/FBG probe can perform substantial progress in this field, as it embeds a dual sensitivity mechanism to refractive index changes (sensitivity up to 266.1 dB/RIU (refractive index units)) that can be exploited in biosensing, while simultaneously having the capability to measure the temperature (sensitivity 9.888 pm/°C), thus providing an intrinsic cross-sensitivity compensation. In addition, a standard FBG analyzer can be used as an interrogator, improving affordability and real-time detection over previous works. The probes have been functionalized with antibodies specific for L1, A27 and A33 vaccinia virus proteins, performing detection of a protein concentration in a scenario compatible with online viral threat detection. Direct detection of wastewater samples shows that the L1-functionalized sensor has a higher response, 9.1–11.3 times higher than A33 and A27, respectively, with a maximum response of up to 1.99 dB and excellent specificity. Dynamic detection in wastewater shows that the sensors have a response over multiple detection cycles, with a sensitivity of 0.024–0.153 dB for each 10-fold increase of concentration.http://www.sciencedirect.com/science/article/pii/S2214180424000813Optical fiber biosensorsSemi-distributed interferometerVirus detectionWastewater-based diagnosticSurface functionalizationVaccinia virus |
| spellingShingle | Albina Abdossova Aina Adilzhankyzy Kuanysh Seitkamal Massimo Olivero Guido Perrone Wilfried Blanc Luca Vangelista Daniele Tosi Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes Sensing and Bio-Sensing Research Optical fiber biosensors Semi-distributed interferometer Virus detection Wastewater-based diagnostic Surface functionalization Vaccinia virus |
| title | Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes |
| title_full | Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes |
| title_fullStr | Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes |
| title_full_unstemmed | Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes |
| title_short | Detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi-distributed FBG-assisted interferometric probes |
| title_sort | detection of vaccinia virus proteins in wastewater environment using biofunctionalized optical fiber semi distributed fbg assisted interferometric probes |
| topic | Optical fiber biosensors Semi-distributed interferometer Virus detection Wastewater-based diagnostic Surface functionalization Vaccinia virus |
| url | http://www.sciencedirect.com/science/article/pii/S2214180424000813 |
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