Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications
In the present study, four <i>Lactobacillus</i> strains (<i>Lacticaseibacillus casei</i> ATCC 393, <i>Lacticaseibacillus casei</i> RC-1, <i>Lactobacillus acidophilus</i> RC-2 and <i>Lacticaseibacillus rhamnosus</i> RC-10) were investigated...
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2025-03-01
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| author | Yulian Tumbarski Ivelina Peykova-Shapkova Mihaela Ivanova Remzi Cholakov Agata Dutkiewicz Krzysztof Grzymajło |
| author_facet | Yulian Tumbarski Ivelina Peykova-Shapkova Mihaela Ivanova Remzi Cholakov Agata Dutkiewicz Krzysztof Grzymajło |
| author_sort | Yulian Tumbarski |
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| description | In the present study, four <i>Lactobacillus</i> strains (<i>Lacticaseibacillus casei</i> ATCC 393, <i>Lacticaseibacillus casei</i> RC-1, <i>Lactobacillus acidophilus</i> RC-2 and <i>Lacticaseibacillus rhamnosus</i> RC-10) were investigated to determine their probiotic potential. The strains were evaluated based on their resistance to simulated upper gastrointestinal conditions, tolerance to bile salts, antimicrobial activity, antibiotic resistance and ability to adhere to intestinal cells. All the strains were resistant to simulated upper gastrointestinal conditions (pH 2.5 + pepsin and pH 7.4 + pancreatin) and exhibited cell recovery rates varying between 74.24 and 87.50% (pH 2.5 + pepsin) and between 93.95 and 98.15% (pH 7.4 + pancreatin) at the 24th h of cultivation. The strains demonstrated resistance to various concentrations of bile salts (0.25, 0.5, 0.75 and 1%) with survival rates > 50% (<i>L. casei</i> ATCC 393 and <i>L. casei</i> RC-1) and >60% (<i>L. acidophilus</i> RC-2 and <i>L. rhamnosus</i> RC-10) in bile salt concentration of 0.25% at the 24th h of incubation. The four <i>Lactobacillus</i> components—<i>Lactobacillus</i> culture (LC), cell biomass (CB) and cell-free supernatant (CFS) exhibited moderate to high antimicrobial activity against six Gram-positive and six Gram-negative bacteria; however, they failed to inhibit the growth of yeasts and fungi tested in the antimicrobial screening. In addition, the neutralized to pH = 7.0 cell-free supernatant (NCFS) of the four strains completely lost its antimicrobial effect. The results for antibiotic susceptibility of four <i>Lactobacillus</i> strains showed that <i>L. casei</i> ATCC 393 was resistant to 11, <i>L. casei</i> RC-1 was resistant to 9, <i>L. acidophilus</i> RC-2 was resistant to 8, and <i>L. rhamnosus</i> RC-10 was resistant to 11 of the total 23 antibiotics tested. The highest degree of adhesion of the studied isolates to the intestinal cell line IPEC-J2 was observed in <i>L. casei</i> RC-1 (39.45%)<i>, L. rhamnosus</i> RC-10 (23.38%) and <i>L. acidophilus</i> RC-2 (19.25%) in comparison with the reference probiotic strain <i>L. casei</i> ATCC 393. Based on the results obtained, the strains studied can be considered as having probiotic potential. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-36c29b99a91a4e0aa70da5ca1ca9b2fb2025-08-20T03:40:43ZengMDPI AGApplied Sciences2076-34172025-03-01156290210.3390/app15062902Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic ApplicationsYulian Tumbarski0Ivelina Peykova-Shapkova1Mihaela Ivanova2Remzi Cholakov3Agata Dutkiewicz4Krzysztof Grzymajło5Department of Microbiology and Biotechnology, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment of Milk and Dairy Products, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment of Milk and Dairy Products, University of Food Technologies, 4002 Plovdiv, BulgariaÖzgazi B. V. Dairy Foods, 4879 AP Etten-Leur, The NetherlandsDepartment of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandIn the present study, four <i>Lactobacillus</i> strains (<i>Lacticaseibacillus casei</i> ATCC 393, <i>Lacticaseibacillus casei</i> RC-1, <i>Lactobacillus acidophilus</i> RC-2 and <i>Lacticaseibacillus rhamnosus</i> RC-10) were investigated to determine their probiotic potential. The strains were evaluated based on their resistance to simulated upper gastrointestinal conditions, tolerance to bile salts, antimicrobial activity, antibiotic resistance and ability to adhere to intestinal cells. All the strains were resistant to simulated upper gastrointestinal conditions (pH 2.5 + pepsin and pH 7.4 + pancreatin) and exhibited cell recovery rates varying between 74.24 and 87.50% (pH 2.5 + pepsin) and between 93.95 and 98.15% (pH 7.4 + pancreatin) at the 24th h of cultivation. The strains demonstrated resistance to various concentrations of bile salts (0.25, 0.5, 0.75 and 1%) with survival rates > 50% (<i>L. casei</i> ATCC 393 and <i>L. casei</i> RC-1) and >60% (<i>L. acidophilus</i> RC-2 and <i>L. rhamnosus</i> RC-10) in bile salt concentration of 0.25% at the 24th h of incubation. The four <i>Lactobacillus</i> components—<i>Lactobacillus</i> culture (LC), cell biomass (CB) and cell-free supernatant (CFS) exhibited moderate to high antimicrobial activity against six Gram-positive and six Gram-negative bacteria; however, they failed to inhibit the growth of yeasts and fungi tested in the antimicrobial screening. In addition, the neutralized to pH = 7.0 cell-free supernatant (NCFS) of the four strains completely lost its antimicrobial effect. The results for antibiotic susceptibility of four <i>Lactobacillus</i> strains showed that <i>L. casei</i> ATCC 393 was resistant to 11, <i>L. casei</i> RC-1 was resistant to 9, <i>L. acidophilus</i> RC-2 was resistant to 8, and <i>L. rhamnosus</i> RC-10 was resistant to 11 of the total 23 antibiotics tested. The highest degree of adhesion of the studied isolates to the intestinal cell line IPEC-J2 was observed in <i>L. casei</i> RC-1 (39.45%)<i>, L. rhamnosus</i> RC-10 (23.38%) and <i>L. acidophilus</i> RC-2 (19.25%) in comparison with the reference probiotic strain <i>L. casei</i> ATCC 393. Based on the results obtained, the strains studied can be considered as having probiotic potential.https://www.mdpi.com/2076-3417/15/6/2902<i>Lactobacillus</i>probiotic strainsprobiotic propertiesprobioticsbiological activities |
| spellingShingle | Yulian Tumbarski Ivelina Peykova-Shapkova Mihaela Ivanova Remzi Cholakov Agata Dutkiewicz Krzysztof Grzymajło Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications Applied Sciences <i>Lactobacillus</i> probiotic strains probiotic properties probiotics biological activities |
| title | Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications |
| title_full | Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications |
| title_fullStr | Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications |
| title_full_unstemmed | Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications |
| title_short | Characterization and Selection of <i>Lactobacillus</i> Strains with Potential Probiotic Applications |
| title_sort | characterization and selection of i lactobacillus i strains with potential probiotic applications |
| topic | <i>Lactobacillus</i> probiotic strains probiotic properties probiotics biological activities |
| url | https://www.mdpi.com/2076-3417/15/6/2902 |
| work_keys_str_mv | AT yuliantumbarski characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications AT ivelinapeykovashapkova characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications AT mihaelaivanova characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications AT remzicholakov characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications AT agatadutkiewicz characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications AT krzysztofgrzymajło characterizationandselectionofilactobacillusistrainswithpotentialprobioticapplications |