Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells

Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells

Summary: KMT2A rearrangements are associated with a poor clinical outcome in infant, pediatric, and adult acute lymphoblastic and myeloid leukemia. Here, we present a protocol to reconstruct chromosomal translocations with different partner genes of KMT2A in vitro. We describe steps for patient-spec...

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Bibliographic Details
Main Authors: Tamara Benz, Patrizia Larghero, Claus Meyer, Marcel Müller, Dörthe Brüggmann, Anna-Elisabeth Hentrich, Frank Louwen, Estelle Erkner, Rahel Fitzel, Corina Schneidawind, Rolf Marschalek
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cancer
Genetics
CRISPR
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006464
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http://www.sciencedirect.com/science/article/pii/S2666166724006464

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