Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification
The objective of this study was to electrophysiologically assess the corticospinal tracts of adult rats and the recovery of motor function of their forelimbs after cervical cord hemisection. Of 39 adult rats used, compound muscle action potentials (CMAPs) of the forelimbs of 15 rats were evaluated,...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2017/7514681 |
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| author | Takumi Takeuchi Masahito Takahashi Kazuhiko Satomi Hideaki Ohne Atsushi Hasegawa Shunsuke Sato Shoichi Ichimura |
| author_facet | Takumi Takeuchi Masahito Takahashi Kazuhiko Satomi Hideaki Ohne Atsushi Hasegawa Shunsuke Sato Shoichi Ichimura |
| author_sort | Takumi Takeuchi |
| collection | DOAJ |
| description | The objective of this study was to electrophysiologically assess the corticospinal tracts of adult rats and the recovery of motor function of their forelimbs after cervical cord hemisection. Of 39 adult rats used, compound muscle action potentials (CMAPs) of the forelimbs of 15 rats were evaluated, before they received left C5 segmental hemisection of the spinal cord, by stimulating the pyramid of the medulla oblongata on one side using an exciting microelectrode. All 15 rats exhibited contralateral electrical activity, but their CMAPs disappeared after hemisection. The remaining 24 rats received hemisection first, and CMAPs of 12 rats were assessed over time to study their recovery time. All of them exhibited electrical activity of the forelimbs in 4 weeks after surgery. The remaining 12 rats received additional right C2 segmental hemisection, and variation of CMAPs between before and after surgery was examined. The right side of the 12 rats that received the additional hemisection exhibited no electrical activity in response to the stimulation of the pyramids on both sides. These results suggest that changes in path between the resected and healthy sides, activation of the ventral corticospinal tracts, and propriospinal neurons were involved in the recovery of motor function after cervical cord injury. |
| format | Article |
| id | doaj-art-336b6be5eaa24d6fb734c2112a40e39c |
| institution | Kabale University |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-336b6be5eaa24d6fb734c2112a40e39c2025-08-20T03:55:15ZengWileyBehavioural Neurology0953-41801875-85842017-01-01201710.1155/2017/75146817514681Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological VerificationTakumi Takeuchi0Masahito Takahashi1Kazuhiko Satomi2Hideaki Ohne3Atsushi Hasegawa4Shunsuke Sato5Shoichi Ichimura6Department of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanDepartment of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanDepartment of Orthopaedic Surgery, Kugayama Hospital, 2-14-20 Kitakarasuyama, Setagaya-ku, Tokyo 157-0061, JapanDepartment of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanDepartment of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanDepartment of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanDepartment of Orthopaedic Surgery, Kyorin University, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-0004, JapanThe objective of this study was to electrophysiologically assess the corticospinal tracts of adult rats and the recovery of motor function of their forelimbs after cervical cord hemisection. Of 39 adult rats used, compound muscle action potentials (CMAPs) of the forelimbs of 15 rats were evaluated, before they received left C5 segmental hemisection of the spinal cord, by stimulating the pyramid of the medulla oblongata on one side using an exciting microelectrode. All 15 rats exhibited contralateral electrical activity, but their CMAPs disappeared after hemisection. The remaining 24 rats received hemisection first, and CMAPs of 12 rats were assessed over time to study their recovery time. All of them exhibited electrical activity of the forelimbs in 4 weeks after surgery. The remaining 12 rats received additional right C2 segmental hemisection, and variation of CMAPs between before and after surgery was examined. The right side of the 12 rats that received the additional hemisection exhibited no electrical activity in response to the stimulation of the pyramids on both sides. These results suggest that changes in path between the resected and healthy sides, activation of the ventral corticospinal tracts, and propriospinal neurons were involved in the recovery of motor function after cervical cord injury.http://dx.doi.org/10.1155/2017/7514681 |
| spellingShingle | Takumi Takeuchi Masahito Takahashi Kazuhiko Satomi Hideaki Ohne Atsushi Hasegawa Shunsuke Sato Shoichi Ichimura Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification Behavioural Neurology |
| title | Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification |
| title_full | Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification |
| title_fullStr | Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification |
| title_full_unstemmed | Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification |
| title_short | Mechanism of Restoration of Forelimb Motor Function after Cervical Spinal Cord Hemisection in Rats: Electrophysiological Verification |
| title_sort | mechanism of restoration of forelimb motor function after cervical spinal cord hemisection in rats electrophysiological verification |
| url | http://dx.doi.org/10.1155/2017/7514681 |
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