Optimization of Amplicon-Based Sequencing for Large-Scale Diagnostics of Known Pome Viruses and Viroids

Optimization of Amplicon-Based Sequencing for Large-Scale Diagnostics of Known Pome Viruses and Viroids

The implementation of reliable methods for the simultaneous detection of multiple viruses is challenging in plant viral diagnosis. In the present study, we report the development and effectiveness of an optimized version of a robust multiplexed PCR (HiPlex) followed by simultaneous targeted amplicon...

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Bibliographic Details
Main Authors: Larissa C. Costa, Xiaojun Hu, Kurt Lamour, Christina Devorshak, Joseph A. Foster, Oscar P. Hurtado-Gonzales
Format: Article
Language:English
Published: The American Phytopathological Society 2024-12-01
Series:PhytoFrontiers
Subjects:
large-scale multiplex PCR
molecular diagnosis of plant viruses
next-generation sequencing
targeted amplicon sequencing
viruses and viroids of pome fruit trees
Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-24-0003-SC
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https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-24-0003-SC

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