Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants
The insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The <i>Tuta absoluta</i> (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, th...
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2024-10-01
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| author | Luo-Hua Shang Xiang-Yun Cai Xiu-Jie Li Yu-Zhou Wang Jin-Da Wang You-Ming Hou |
| author_facet | Luo-Hua Shang Xiang-Yun Cai Xiu-Jie Li Yu-Zhou Wang Jin-Da Wang You-Ming Hou |
| author_sort | Luo-Hua Shang |
| collection | DOAJ |
| description | The insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The <i>Tuta absoluta</i> (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, the relationship between gut microflora and host adaption of <i>T. absoluta</i> remains to be known. In this study, we first compared the survival adaptability of <i>T. absoluta</i> feeding with two host plants (tomatoes and potatoes). The <i>T. absoluta</i> completed the generation cycle by feeding on the leaves of both plants. However, the larvae feeding on tomato leaves have shorter larvae durations, longer adult durations, and a greater number of egg production per female. After Single Molecular Real-Time (SMRT) sequencing, according to the LDA Effect Size (LEfSe) analysis, the gut bacterial biomarker of <i>T. absoluta</i> fed on tomato was <i>Enterobacter cloacae</i> and the gut bacterial biomarker of <i>T. absoluta</i> fed on potatoes was <i>Staphylococcus gallinarum</i> and <i>Enterococcus gallinarum</i>. Furthermore, a total of 6 and 7 culturable bacteria were isolated from the guts of tomato- and potato-treated <i>T. absoluta</i>, respectively. However, the isolated strains included bacterial biomarkers <i>E. cloacae</i> and <i>S. gallinarum</i> but not <i>E. gallinarum</i>. In addition, different stains bacterial biomarkers on <i>T. absoluta</i> feeding selection were also studied. <i>E. cloacae</i> enhanced the host preference of the SLTA (<i>T. absoluta</i> of tomato strain) for tomato but had no impact on STTA (<i>T. absoluta</i> of potato strain). <i>S. gallinarum</i> improved the host preference of STTA to a potato but did not affect SLTA. The results showed that the gut bacteria of <i>T. absoluta</i> were affected by exposure to different host plants, and the bacterial biomarkers played an important role in host adaptability. This study not only deepens our understanding of gut bacteria-mediated insect-plant interactions but also provides theoretical support for the development of environmentally friendly and effective agricultural pest control methods. |
| format | Article |
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| spelling | doaj-art-31fdb6b53ccc4c00b0ee2d0b51ed88222025-08-20T02:11:05ZengMDPI AGInsects2075-44502024-10-01151079510.3390/insects15100795Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host PlantsLuo-Hua Shang0Xiang-Yun Cai1Xiu-Jie Li2Yu-Zhou Wang3Jin-Da Wang4You-Ming Hou5State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The <i>Tuta absoluta</i> (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, the relationship between gut microflora and host adaption of <i>T. absoluta</i> remains to be known. In this study, we first compared the survival adaptability of <i>T. absoluta</i> feeding with two host plants (tomatoes and potatoes). The <i>T. absoluta</i> completed the generation cycle by feeding on the leaves of both plants. However, the larvae feeding on tomato leaves have shorter larvae durations, longer adult durations, and a greater number of egg production per female. After Single Molecular Real-Time (SMRT) sequencing, according to the LDA Effect Size (LEfSe) analysis, the gut bacterial biomarker of <i>T. absoluta</i> fed on tomato was <i>Enterobacter cloacae</i> and the gut bacterial biomarker of <i>T. absoluta</i> fed on potatoes was <i>Staphylococcus gallinarum</i> and <i>Enterococcus gallinarum</i>. Furthermore, a total of 6 and 7 culturable bacteria were isolated from the guts of tomato- and potato-treated <i>T. absoluta</i>, respectively. However, the isolated strains included bacterial biomarkers <i>E. cloacae</i> and <i>S. gallinarum</i> but not <i>E. gallinarum</i>. In addition, different stains bacterial biomarkers on <i>T. absoluta</i> feeding selection were also studied. <i>E. cloacae</i> enhanced the host preference of the SLTA (<i>T. absoluta</i> of tomato strain) for tomato but had no impact on STTA (<i>T. absoluta</i> of potato strain). <i>S. gallinarum</i> improved the host preference of STTA to a potato but did not affect SLTA. The results showed that the gut bacteria of <i>T. absoluta</i> were affected by exposure to different host plants, and the bacterial biomarkers played an important role in host adaptability. This study not only deepens our understanding of gut bacteria-mediated insect-plant interactions but also provides theoretical support for the development of environmentally friendly and effective agricultural pest control methods.https://www.mdpi.com/2075-4450/15/10/795insect gut bacteriaSMRTbacterial biomarker<i>Tuta absoluta</i>host adaptability |
| spellingShingle | Luo-Hua Shang Xiang-Yun Cai Xiu-Jie Li Yu-Zhou Wang Jin-Da Wang You-Ming Hou Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants Insects insect gut bacteria SMRT bacterial biomarker <i>Tuta absoluta</i> host adaptability |
| title | Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants |
| title_full | Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants |
| title_fullStr | Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants |
| title_full_unstemmed | Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants |
| title_short | Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants |
| title_sort | role of gut bacteria in enhancing host adaptation of i tuta absoluta i to different host plants |
| topic | insect gut bacteria SMRT bacterial biomarker <i>Tuta absoluta</i> host adaptability |
| url | https://www.mdpi.com/2075-4450/15/10/795 |
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