High quality RNA extraction from Pseudomonas aeruginosa and other bacteria with a novel rapid and cost-effective method

High quality RNA extraction from Pseudomonas aeruginosa and other bacteria with a novel rapid and cost-effective method

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS for the extraction of intact RNA from Pseudomonas aeruginosa and compared its performance to established rapid techniques like RNAsnap and CiAR. The RNA integrity, yi...

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Bibliographic Details
Main Authors: Ivan Stoikov, Ivan Nikolaev Ivanov, Deyan Donchev, Elina Dobreva, Rumyana Hristova, Stefana Sabtcheva
Format: Article
Language:English
Published: Taylor & Francis Group 2023-03-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Pseudomonas
RNA extraction
RIN
gene expression
Gram-positive
RT-PCR
Online Access:https://www.tandfonline.com/doi/10.1080/13102818.2023.2234502
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https://www.tandfonline.com/doi/10.1080/13102818.2023.2234502

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