High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast
Lignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However, the challenges associated with the co-utilization of xylose and glucose often hinders the efficiency o...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X24001613 |
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| author | Xin Ni Jingjing Li Wei Yu Fan Bai Zongbao K. Zhao Jiaoqi Gao Fan Yang Yongjin J. Zhou |
| author_facet | Xin Ni Jingjing Li Wei Yu Fan Bai Zongbao K. Zhao Jiaoqi Gao Fan Yang Yongjin J. Zhou |
| author_sort | Xin Ni |
| collection | DOAJ |
| description | Lignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However, the challenges associated with the co-utilization of xylose and glucose often hinders the efficiency of lignocellulose bioconversion. Here, we engineered yeast Ogataea polymorpha to effectively produce free fatty acids from lignocellulose. The non-oxidative branch of the pentose phosphate pathway, and the adaptive expression levels of xylose metabolic pathway genes XYL1, XYL2 and XYL3, were systematically optimized. In addition, the introduction of xylose transporter and global regulation of transcription factors achieved synchronous co-utilization of glucose and xylose. The engineered strain produced 11.2 g/L FFAs from lignocellulose hydrolysates, with a yield of up to 0.054 g/g. This study demonstrated that metabolic rewiring of xylose metabolism could support the efficient co-utilization of glucose and xylose from lignocellulosic resources, which may provide theoretical reference for lignocellulose biorefinery. |
| format | Article |
| id | doaj-art-2ec1cc52fa73466f9bf4412019039962 |
| institution | Kabale University |
| issn | 2405-805X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj-art-2ec1cc52fa73466f9bf44120190399622025-01-08T04:53:06ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2025-06-01102401409High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeastXin Ni0Jingjing Li1Wei Yu2Fan Bai3Zongbao K. Zhao4Jiaoqi Gao5Fan Yang6Yongjin J. Zhou7School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, PR China; Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR ChinaDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR ChinaDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR ChinaDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR ChinaMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, PR ChinaDivision of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China; Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, PR China; Corresponding author. Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China.School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, PR China; Corresponding author.Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China; CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR ChinaLignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However, the challenges associated with the co-utilization of xylose and glucose often hinders the efficiency of lignocellulose bioconversion. Here, we engineered yeast Ogataea polymorpha to effectively produce free fatty acids from lignocellulose. The non-oxidative branch of the pentose phosphate pathway, and the adaptive expression levels of xylose metabolic pathway genes XYL1, XYL2 and XYL3, were systematically optimized. In addition, the introduction of xylose transporter and global regulation of transcription factors achieved synchronous co-utilization of glucose and xylose. The engineered strain produced 11.2 g/L FFAs from lignocellulose hydrolysates, with a yield of up to 0.054 g/g. This study demonstrated that metabolic rewiring of xylose metabolism could support the efficient co-utilization of glucose and xylose from lignocellulosic resources, which may provide theoretical reference for lignocellulose biorefinery.http://www.sciencedirect.com/science/article/pii/S2405805X24001613LignocelluloseXyloseFatty acidsOgataea polymorphaBiorefinery |
| spellingShingle | Xin Ni Jingjing Li Wei Yu Fan Bai Zongbao K. Zhao Jiaoqi Gao Fan Yang Yongjin J. Zhou High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast Synthetic and Systems Biotechnology Lignocellulose Xylose Fatty acids Ogataea polymorpha Biorefinery |
| title | High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast |
| title_full | High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast |
| title_fullStr | High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast |
| title_full_unstemmed | High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast |
| title_short | High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast |
| title_sort | high level production of free fatty acids from lignocellulose hydrolysate by co utilizing glucose and xylose in yeast |
| topic | Lignocellulose Xylose Fatty acids Ogataea polymorpha Biorefinery |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X24001613 |
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