Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium
This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes...
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College of Veterinary Medicine, University of Basrah, Iraq
2024-09-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_184793_bcb288d6021245b4756605cf60712be3.pdf |
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| author | Abdul-Samad Uleiwi Hassan jian ibraheem |
| author_facet | Abdul-Samad Uleiwi Hassan jian ibraheem |
| author_sort | Abdul-Samad Uleiwi Hassan |
| collection | DOAJ |
| description | This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. |
| format | Article |
| id | doaj-art-2e94bde63a3c48deb02e8d22e11bbd22 |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-2e94bde63a3c48deb02e8d22e11bbd222025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562024-09-01233556210.23975/bjvr.2024.147966.1071184793Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs MediumAbdul-Samad Uleiwi Hassan0jian ibraheem1Al-Forat Al-Awsat Technical University, Health & Medical Techniques College, Medical Laboratories Techniques Department.Middle Technical University/ Health & Medical Techniques College, Anaesthesia Techniques DepartmentThis project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates.https://bjvr.uobasrah.edu.iq/article_184793_bcb288d6021245b4756605cf60712be3.pdfb. indicusoocytesin-vitro maturationco2 gas stagem-pbs |
| spellingShingle | Abdul-Samad Uleiwi Hassan jian ibraheem Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium Basrah Journal of Veterinary Research b. indicus oocytes in-vitro maturation co2 gas stage m-pbs |
| title | Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium |
| title_full | Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium |
| title_fullStr | Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium |
| title_full_unstemmed | Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium |
| title_short | Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium |
| title_sort | applying modified 2000 in vitro technique for maturation of b indicus oocytes by using plastic test tubes supplemented with m pbs medium |
| topic | b. indicus oocytes in-vitro maturation co2 gas stage m-pbs |
| url | https://bjvr.uobasrah.edu.iq/article_184793_bcb288d6021245b4756605cf60712be3.pdf |
| work_keys_str_mv | AT abdulsamaduleiwihassan applyingmodified2000invitrotechniqueformaturationofbindicusoocytesbyusingplastictesttubessupplementedwithmpbsmedium AT jianibraheem applyingmodified2000invitrotechniqueformaturationofbindicusoocytesbyusingplastictesttubessupplementedwithmpbsmedium |