Applying Modified 2000 In Vitro Technique for Maturation of B. Indicus Oocytes by Using Plastic Test Tubes Supplemented with M-Pbs Medium
This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2024-09-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_184793_bcb288d6021245b4756605cf60712be3.pdf |
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| Summary: | This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. This project was conducted in Health and Medical Technical College's laboratories of Al-Forat Al-Awsat Technical University in 2021. The study was dealt 370 oocytes collected from 19 three years old cows by using dissecting process. The gathered oocytes were transferred hastily to a test tubes contains m-PBS prelude for incubation by modified in vitro technique 2000, which supplemented with 5% Co2 gas state for 26 hour at 37oc. The gained consequences led to 20% maturation occurred for the second metaphase after applying m-PBS and staining with orcein stain. Statistics supports our hypothesis. The outcomes for this study is that incubation of B. indicus oocytes in vitro by modified technique 2000 with using m-PBS culture media will established a success and promising in maturation rates. |
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| ISSN: | 1813-8497 2410-8456 |