Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid
A mixed copper complex with deprotonated nalidixic acid (nal) and histamine (hsm) was synthesized and characterized by FTIR, UV-Vis, elemental analysis, and conductivity. The crystal structure of [Cu(hsm)(nal)H2O]Cl·3H2O (chn) showed a pentacoordinated cooper(II) in a square pyramidal geometry surro...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2009/603651 |
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| author | Egla Yareth Bivián-Castro Mercedes G. López Mario Pedraza-Reyes Sylvain Bernès Guillermo Mendoza-Díaz |
| author_facet | Egla Yareth Bivián-Castro Mercedes G. López Mario Pedraza-Reyes Sylvain Bernès Guillermo Mendoza-Díaz |
| author_sort | Egla Yareth Bivián-Castro |
| collection | DOAJ |
| description | A mixed copper complex with deprotonated nalidixic acid (nal) and histamine (hsm) was synthesized and characterized by FTIR, UV-Vis, elemental analysis, and conductivity. The crystal structure of [Cu(hsm)(nal)H2O]Cl·3H2O (chn) showed a pentacoordinated cooper(II) in a square pyramidal geometry surrounded by two N atoms from hsm, two O atoms from the quinolone, and one apical water oxygen.
Alteration of bacterial DNA structure and/or associated functions in vivo by [Cu(hsm)(nal)H2O]Cl·3H2O was demonstrated by the induction of a recA-lacZ fusion integrated at the amyE locus of a recombinant Bacillus subtilis strain. Results from circular dichroism and denaturation of calf thymus DNA (CT-DNA) suggested that increased amounts of copper complex were able to stabilize the double helix of DNA in vitro mainly by formation of hydrogen bonds between chn and the sugars of DNA minor groove. In vivo and in vitro biological activities of the chn complex were compared with the chemical nuclease [Cu(phen)(nal)H2O]NO3·3H2O (cpn) where phen is phenanthroline. |
| format | Article |
| id | doaj-art-2e7924e48e75483a9e6af4ad0bbeb5c4 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-2e7924e48e75483a9e6af4ad0bbeb5c42025-08-20T03:55:07ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2009-01-01200910.1155/2009/603651603651Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic AcidEgla Yareth Bivián-Castro0Mercedes G. López1Mario Pedraza-Reyes2Sylvain Bernès3Guillermo Mendoza-Díaz4Departamento de Química, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, 36050 Guanajuato, GTO, MexicoDepartamento de Biotecnología y Bioquímica, Cinvestav-IPN Unidad Irapuato, Km.9.6 Libramiento Norte, Carr. Irapuato-León, 36500 Irapuato, GTO, MexicoDepartamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, 36050 Guanajuato, GTO, MexicoDEP Facultad de Ciencias Químicas, UANL, Guerrero y Progreso s/n, Col. Treviño, 64570 Monterrey, NL, MexicoDepartamento de Química, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, 36050 Guanajuato, GTO, MexicoA mixed copper complex with deprotonated nalidixic acid (nal) and histamine (hsm) was synthesized and characterized by FTIR, UV-Vis, elemental analysis, and conductivity. The crystal structure of [Cu(hsm)(nal)H2O]Cl·3H2O (chn) showed a pentacoordinated cooper(II) in a square pyramidal geometry surrounded by two N atoms from hsm, two O atoms from the quinolone, and one apical water oxygen. Alteration of bacterial DNA structure and/or associated functions in vivo by [Cu(hsm)(nal)H2O]Cl·3H2O was demonstrated by the induction of a recA-lacZ fusion integrated at the amyE locus of a recombinant Bacillus subtilis strain. Results from circular dichroism and denaturation of calf thymus DNA (CT-DNA) suggested that increased amounts of copper complex were able to stabilize the double helix of DNA in vitro mainly by formation of hydrogen bonds between chn and the sugars of DNA minor groove. In vivo and in vitro biological activities of the chn complex were compared with the chemical nuclease [Cu(phen)(nal)H2O]NO3·3H2O (cpn) where phen is phenanthroline.http://dx.doi.org/10.1155/2009/603651 |
| spellingShingle | Egla Yareth Bivián-Castro Mercedes G. López Mario Pedraza-Reyes Sylvain Bernès Guillermo Mendoza-Díaz Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid Bioinorganic Chemistry and Applications |
| title | Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid |
| title_full | Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid |
| title_fullStr | Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid |
| title_full_unstemmed | Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid |
| title_short | Synthesis, Characterization, and Biological Activity Studies of Copper(II) Mixed Compound with Histamine and Nalidixic Acid |
| title_sort | synthesis characterization and biological activity studies of copper ii mixed compound with histamine and nalidixic acid |
| url | http://dx.doi.org/10.1155/2009/603651 |
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