Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae

Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae

In synthetic biology, microbial chassis including yeast Saccharomyces cerevisiae are iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering tools are developed and optimised to facilitate strain construction but are often incompatible with each other due to shared r...

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Bibliographic Details
Main Authors: Liam McDonnell, Samuel Evans, Zeyu Lu, Mitch Suchoronczak, Jonah Leighton, Eugene Ordeniza, Blake Ritchie, Nik Valado, Niamh Walsh, James Antoney, Chengqiang Wang, Carlos Horacio Luna-Flores, Colin Scott, Robert Speight, Claudia E. Vickers, Bingyin Peng
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
Metabolic engineering
Genetic circuit
Genome engineering
Synthetic biology
Yeast engineering
Promoter engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X2400098X
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