Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3

Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3

Abstract We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and...

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Bibliographic Details
Main Authors: Hsiu-Chun Chuang, Ruidong Li, Huang Huang, Szu-Wen Liu, Christine Wan, Subhra Chaudhuri, Lili Yue, Terence Wong, Venina Dominical, Randy Yen, Olivia Ngo, Nam Bui, Hubert Stoppler, Tangsheng Yi, Silpa Suthram, Li Li, Kai-Hui Sun
Format: Article
Language:English
Published: BMC 2024-11-01
Series:BMC Genomics
Subjects:
High-throughput Smart-seq3
10X
Single-cell RNA sequencing
Single-cell TCR sequencing
Online Access:https://doi.org/10.1186/s12864-024-11036-0
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https://doi.org/10.1186/s12864-024-11036-0

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