Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies

The present study focuses on research findings related to the development and assessment of thiadiazole-linked thiazole derivatives as promising anti-tubercular agents. We present the synthesis data of eleven new compounds (4a-4k) and confirm their structures using spectroscopic techniques. Subseque...

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Main Authors: Samin A. Shaikh, Shivaji R. Labhade, Raju R. Kale, Prajakta Y. Pachorkar, Rohan J. Meshram, Kamlesh S. Jain, Hrishikesh S. Labhade, Dipak D. Bhanushali, Rahul A. More, Charushila K. Nerkar, Santosh S. Chobe, Aniket N. Marathe, Satish N. Wakchaure, Deepak R. Boraste
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:European Journal of Medicinal Chemistry Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772417424000554
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author Samin A. Shaikh
Shivaji R. Labhade
Raju R. Kale
Prajakta Y. Pachorkar
Rohan J. Meshram
Kamlesh S. Jain
Hrishikesh S. Labhade
Dipak D. Bhanushali
Rahul A. More
Charushila K. Nerkar
Santosh S. Chobe
Aniket N. Marathe
Satish N. Wakchaure
Deepak R. Boraste
author_facet Samin A. Shaikh
Shivaji R. Labhade
Raju R. Kale
Prajakta Y. Pachorkar
Rohan J. Meshram
Kamlesh S. Jain
Hrishikesh S. Labhade
Dipak D. Bhanushali
Rahul A. More
Charushila K. Nerkar
Santosh S. Chobe
Aniket N. Marathe
Satish N. Wakchaure
Deepak R. Boraste
author_sort Samin A. Shaikh
collection DOAJ
description The present study focuses on research findings related to the development and assessment of thiadiazole-linked thiazole derivatives as promising anti-tubercular agents. We present the synthesis data of eleven new compounds (4a-4k) and confirm their structures using spectroscopic techniques. Subsequently, the compounds were screened for their anti-tuberculosis activities against M. tuberculosis H37Ra. The results demonstrated that compounds 3 and 4b exhibited minimum inhibitory concentration (MIC) of 3.90 μg/mL and 7.81 μg/mL, respectively. In-vitro, studies for few compounds exhibited high antioxidant activity against DPPH and OH radical scavengers along with minimal to no cytotoxicity against RBCs which is a promising result. Investigation of molecular docked conformations revealed different molecular interactions such as hydrogen bonds, halogen bonds, and interactions involving Pi electron cloud. The study sheds light on conserved interactions with residues like Met131, Val163, His90 and Gln161 from the tubercular MCAT enzyme. Interestingly, the synthetic chemistry reveals that the employment of tetra-n-butylammonium bromide (TBAB) plays a crucial role for N-butylation and it also expedites the reaction in tetrahydrofuran solvent.
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spelling doaj-art-2b41aece8a7c44a79eb3205b51c8e6d92024-12-05T05:21:48ZengElsevierEuropean Journal of Medicinal Chemistry Reports2772-41742024-12-0112100183Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studiesSamin A. Shaikh0Shivaji R. Labhade1Raju R. Kale2Prajakta Y. Pachorkar3Rohan J. Meshram4Kamlesh S. Jain5Hrishikesh S. Labhade6Dipak D. Bhanushali7Rahul A. More8Charushila K. Nerkar9Santosh S. Chobe10Aniket N. Marathe11Satish N. Wakchaure12Deepak R. Boraste13Department of Chemistry, KTHM College, Gangapur Road, Nashik, Maharashtra, India; Department of Chemistry, Kr. V. N. Naik Shikshan Prasarak Sanstha's Arts, Commerce and Science College, Canada Corner, Nashik, Maharashtra, India; Corresponding author. Department of Chemistry, KTHM College, Gangapur Road, Nashik, Maharashtra, India.Department of Chemistry, KTHM College, Gangapur Road, Nashik, Maharashtra, IndiaDepartment of Chemistry, KTHM College, Gangapur Road, Nashik, Maharashtra, IndiaDepartment of Microbiology, KTHM College, Gangapur Road, Nashik, Maharashtra, IndiaBioinformatics Centre, Savitribai Phule Pune University, Pune, Maharashtra, IndiaDepartment of Chemistry, Kr. V. N. Naik Shikshan Prasarak Sanstha's Arts, Commerce and Science College, Canada Corner, Nashik, Maharashtra, IndiaDepartment of Chemistry, KTHM College, Gangapur Road, Nashik, Maharashtra, IndiaDepartment of Chemistry, Kr. V. N. Naik Shikshan Prasarak Sanstha's Arts, Commerce and Science College, Canada Corner, Nashik, Maharashtra, IndiaDepartment of Microbiology, Dayanand Science College, Latur, Maharashtra, IndiaDepartment of Chemistry, M.G. V's Loknete Vyankatrao Hiray, Arts, Science and Commerce College, Panchavati, Nashik, Maharashtra, IndiaDepartment of Chemistry, M.G. V's Loknete Vyankatrao Hiray, Arts, Science and Commerce College, Panchavati, Nashik, Maharashtra, IndiaBioinformatics Centre, Savitribai Phule Pune University, Pune, Maharashtra, IndiaDepartment of Synthetic R & D, Delta Finochem Private Limited, Gat No. 350, Village Wadivarhe, Tal-Igatpuri, Nashik, Maharashtra, IndiaG. E. Society's, R.N.C Arts, J.D.B Commerce, and N.S.C. Science College, Nashik Road, Nashik, 422101, Maharashtra, IndiaThe present study focuses on research findings related to the development and assessment of thiadiazole-linked thiazole derivatives as promising anti-tubercular agents. We present the synthesis data of eleven new compounds (4a-4k) and confirm their structures using spectroscopic techniques. Subsequently, the compounds were screened for their anti-tuberculosis activities against M. tuberculosis H37Ra. The results demonstrated that compounds 3 and 4b exhibited minimum inhibitory concentration (MIC) of 3.90 μg/mL and 7.81 μg/mL, respectively. In-vitro, studies for few compounds exhibited high antioxidant activity against DPPH and OH radical scavengers along with minimal to no cytotoxicity against RBCs which is a promising result. Investigation of molecular docked conformations revealed different molecular interactions such as hydrogen bonds, halogen bonds, and interactions involving Pi electron cloud. The study sheds light on conserved interactions with residues like Met131, Val163, His90 and Gln161 from the tubercular MCAT enzyme. Interestingly, the synthetic chemistry reveals that the employment of tetra-n-butylammonium bromide (TBAB) plays a crucial role for N-butylation and it also expedites the reaction in tetrahydrofuran solvent.http://www.sciencedirect.com/science/article/pii/S2772417424000554Anti-tuberculosisThiazoleTetra-n-butylammonium bromideThiadiazole
spellingShingle Samin A. Shaikh
Shivaji R. Labhade
Raju R. Kale
Prajakta Y. Pachorkar
Rohan J. Meshram
Kamlesh S. Jain
Hrishikesh S. Labhade
Dipak D. Bhanushali
Rahul A. More
Charushila K. Nerkar
Santosh S. Chobe
Aniket N. Marathe
Satish N. Wakchaure
Deepak R. Boraste
Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
European Journal of Medicinal Chemistry Reports
Anti-tuberculosis
Thiazole
Tetra-n-butylammonium bromide
Thiadiazole
title Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
title_full Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
title_fullStr Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
title_full_unstemmed Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
title_short Thiadiazole-thiazole derivatives as potent anti-tubercular agents: Synthesis, biological evaluation, and In silico docking studies
title_sort thiadiazole thiazole derivatives as potent anti tubercular agents synthesis biological evaluation and in silico docking studies
topic Anti-tuberculosis
Thiazole
Tetra-n-butylammonium bromide
Thiadiazole
url http://www.sciencedirect.com/science/article/pii/S2772417424000554
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