Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi
Soluble β-glucan from mushroom source had good biological activity, in order to develop and utilize β-glucan (CMBG) in Cordyceps chrysanthemi, this study used Cordyceps chrysanthemi substrate as raw material, and was purified by proteolytic enzyme digestion, adsorption of macroporous resin, and chro...
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| author | Yexin LIN Guangyu YAN Yaru WANG Hua FANG Hui CHEN Zhuan HONG |
| author_facet | Yexin LIN Guangyu YAN Yaru WANG Hua FANG Hui CHEN Zhuan HONG |
| author_sort | Yexin LIN |
| collection | DOAJ |
| description | Soluble β-glucan from mushroom source had good biological activity, in order to develop and utilize β-glucan (CMBG) in Cordyceps chrysanthemi, this study used Cordyceps chrysanthemi substrate as raw material, and was purified by proteolytic enzyme digestion, adsorption of macroporous resin, and chromatography of DEAE ion-exchange columns, and the decolorization rate and β-glucan retention rate were weighted and scored to feel out the establishment of the optimal purification process conditions. The obtained β-glucan was structurally analyzed and its in vitro hypoglycemic activity was investigated. The results showed that the macroporous resin HPD-750 had the best adsorption effect, and the optimal conditions for adsorption were as follows: up-sample flow rate of 5 mL/min, up-sample concentration of 4 mg/mL, up-sample volume of 400 mL, and mass of the resin of 40 g. Two β-glucan fractions, CMBG-1 and CMBG-2, with relative molecular weights of 13.57×104 Da and 14.36×104 Da, respectively, were isolated using a DEAE-FF cellulose exchange column. Both consisted of three monosaccharides, Gal, Glc, and Man, with molar ratios of 1.28:5.27:1.01 and 0.71:4.43:0.80, respectively. The Congo red analysis showed that CMBG-1 had a triple-helix structure, while CMBG-2 had no triple-helix structure, and the inhibition rates of both of them on α-amylase and α-glucosidase were positively correlated with the concentrations of the samples, and the IC50 of their inhibition on α-amylase were 2.68 and 4.32 mg/mL, and those on α-glucosidase were 2.37 and 3.01 mg/mL, respectively. The hypoglycemic activity of CMBG-1, which possessed a triple-helix structure, was significantly higher than that of CMBG-2, which did not possess a triple-helix structure, and it had good prospects for the development of functional foods. |
| format | Article |
| id | doaj-art-28cf05f728d3460c94e6d2c2d8fbcecd |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-28cf05f728d3460c94e6d2c2d8fbcecd2025-01-10T06:49:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-01462556310.13386/j.issn1002-0306.20240200672024020067-2Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemiYexin LIN0Guangyu YAN1Yaru WANG2Hua FANG3Hui CHEN4Zhuan HONG5College of Advanced Manufacturing, Fuzhou University, Quanzhou 362200, Fujian, ChinaXiamen Ocean Vocational College, Xiamen 361000, Fujian, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350001, Fujian, ChinaTechnology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, ChinaTechnology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, ChinaTechnology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, ChinaSoluble β-glucan from mushroom source had good biological activity, in order to develop and utilize β-glucan (CMBG) in Cordyceps chrysanthemi, this study used Cordyceps chrysanthemi substrate as raw material, and was purified by proteolytic enzyme digestion, adsorption of macroporous resin, and chromatography of DEAE ion-exchange columns, and the decolorization rate and β-glucan retention rate were weighted and scored to feel out the establishment of the optimal purification process conditions. The obtained β-glucan was structurally analyzed and its in vitro hypoglycemic activity was investigated. The results showed that the macroporous resin HPD-750 had the best adsorption effect, and the optimal conditions for adsorption were as follows: up-sample flow rate of 5 mL/min, up-sample concentration of 4 mg/mL, up-sample volume of 400 mL, and mass of the resin of 40 g. Two β-glucan fractions, CMBG-1 and CMBG-2, with relative molecular weights of 13.57×104 Da and 14.36×104 Da, respectively, were isolated using a DEAE-FF cellulose exchange column. Both consisted of three monosaccharides, Gal, Glc, and Man, with molar ratios of 1.28:5.27:1.01 and 0.71:4.43:0.80, respectively. The Congo red analysis showed that CMBG-1 had a triple-helix structure, while CMBG-2 had no triple-helix structure, and the inhibition rates of both of them on α-amylase and α-glucosidase were positively correlated with the concentrations of the samples, and the IC50 of their inhibition on α-amylase were 2.68 and 4.32 mg/mL, and those on α-glucosidase were 2.37 and 3.01 mg/mL, respectively. The hypoglycemic activity of CMBG-1, which possessed a triple-helix structure, was significantly higher than that of CMBG-2, which did not possess a triple-helix structure, and it had good prospects for the development of functional foods.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020067cordyceps militaris β-glucanseparation and purificationstructural analysishypoglycemic activity |
| spellingShingle | Yexin LIN Guangyu YAN Yaru WANG Hua FANG Hui CHEN Zhuan HONG Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi Shipin gongye ke-ji cordyceps militaris β-glucan separation and purification structural analysis hypoglycemic activity |
| title | Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi |
| title_full | Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi |
| title_fullStr | Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi |
| title_full_unstemmed | Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi |
| title_short | Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi |
| title_sort | isolation and purification structural characterization hypoglycemic activity of soluble β glucan from cordyceps chrysanthemi |
| topic | cordyceps militaris β-glucan separation and purification structural analysis hypoglycemic activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024020067 |
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