Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital
Introduction: The incidence of Candida infections is on the rise with the increase in number of immuno-compromised patients due to excessive use of immunosuppressive drugs as well as the use of medical and surgical interventions. Materials and Methods: A cross-sectional observational study was condu...
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Al Ameen Medical College
2025-01-01
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| Series: | Al Ameen Journal of Medical Sciences |
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| Online Access: | https://ajms.alameenmedical.org/ArticlePDFs/8%20AJMS%20V18.N1.2025%20p%2042-46.pdf |
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| author | Paulami De Rehena Sarkar Raja Ray Kumkum Bhattacharyya Hasina Banu Hirak Jyoti Raj |
| author_facet | Paulami De Rehena Sarkar Raja Ray Kumkum Bhattacharyya Hasina Banu Hirak Jyoti Raj |
| author_sort | Paulami De |
| collection | DOAJ |
| description | Introduction: The incidence of Candida infections is on the rise with the increase in number of immuno-compromised patients due to excessive use of immunosuppressive drugs as well as the use of medical and surgical interventions. Materials and Methods: A cross-sectional observational study was conducted in a tertiary care hospital from February 2021- August 2022. All Candida isolates from various clinical samples during the study period were processed for identification and susceptibility testing by both Automated & Conventional methods with E-strip. Data analysed by using Microsoft excel spreadsheet and then analyzed by SPSS and Graph PadPrism version 5. Result: Total Candida isolates were 100, among them near about half (43%) of the isolates were obtained from critical care unit followed by thoracic unit (17%), Ward (16%), trauma centre (15%), high dependency (7%) and 2% from outdoor. Maximum number of isolates came from central line catheter 43, followed by blood 19. other samples from where candida were isolated are urine 13, endotracheal aspirate 9, Pleural fluid 7, Bronchoalveolar lavage 3, Cerebrospinal Fluid 3 and Wound 3. Isolated pathogens were Candida albicans. Candida tropicalis, Candida parapsilosis, Candida glabrata, Candida auris, Candida guilliermondii , Candida haemulonii, Candida ciferrii, Candida famata, Candida duobushaemulonii. The association between isolated Candida and from which samples they are isolated were statistically significant with ‘P’ value <0.0001 and Chi-square value: 284.4447. Conclusion: The increasing incidence of HIV infections, increasing incidence of organ transplantation, wide spread use of antibiotics and use of immunosuppressive agents are the important causative factors responsible for the increasing incidence of Candida infections. Furthermore, due to increasing resistance to various antifungal agents Candida has assumed greater clinical importance. This observation of the present study is in consonance with the changing trend seen both in India and across the world. |
| format | Article |
| id | doaj-art-28520522501142f5ba372a81871f0ec7 |
| institution | Kabale University |
| issn | 0974-1143 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Al Ameen Medical College |
| record_format | Article |
| series | Al Ameen Journal of Medical Sciences |
| spelling | doaj-art-28520522501142f5ba372a81871f0ec72025-01-11T12:20:10ZengAl Ameen Medical CollegeAl Ameen Journal of Medical Sciences0974-11432025-01-0118014246Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospitalPaulami De0Rehena Sarkar1Raja Ray2Kumkum Bhattacharyya3Hasina Banu4Hirak Jyoti Raj5Department of Microbiology, Sarat Chandra Chatterjee Govt. Medical College and Hospital, Uluberia, Howrah-711315, West Bengal, IndiaDepartment of Microbiology, IPGME&R-SSKM Hospital, 244 AJC Bose Road, Kolkata-700020, West Bengal, IndiaDepartment of Microbiology, IPGME&R-SSKM Hospital, 244 AJC Bose Road, Kolkata-700020, West Bengal, IndiaDepartment of Microbiology, IPGME&R-SSKM Hospital, 244 AJC Bose Road, Kolkata-700020, West Bengal, IndiaDepartment of Microbiology, IPGME&R-SSKM Hospital, 244 AJC Bose Road, Kolkata-700020, West Bengal, IndiaDepartment of Microbiology, IPGME&R-SSKM Hospital, 244 AJC Bose Road, Kolkata-700020, West Bengal, IndiaIntroduction: The incidence of Candida infections is on the rise with the increase in number of immuno-compromised patients due to excessive use of immunosuppressive drugs as well as the use of medical and surgical interventions. Materials and Methods: A cross-sectional observational study was conducted in a tertiary care hospital from February 2021- August 2022. All Candida isolates from various clinical samples during the study period were processed for identification and susceptibility testing by both Automated & Conventional methods with E-strip. Data analysed by using Microsoft excel spreadsheet and then analyzed by SPSS and Graph PadPrism version 5. Result: Total Candida isolates were 100, among them near about half (43%) of the isolates were obtained from critical care unit followed by thoracic unit (17%), Ward (16%), trauma centre (15%), high dependency (7%) and 2% from outdoor. Maximum number of isolates came from central line catheter 43, followed by blood 19. other samples from where candida were isolated are urine 13, endotracheal aspirate 9, Pleural fluid 7, Bronchoalveolar lavage 3, Cerebrospinal Fluid 3 and Wound 3. Isolated pathogens were Candida albicans. Candida tropicalis, Candida parapsilosis, Candida glabrata, Candida auris, Candida guilliermondii , Candida haemulonii, Candida ciferrii, Candida famata, Candida duobushaemulonii. The association between isolated Candida and from which samples they are isolated were statistically significant with ‘P’ value <0.0001 and Chi-square value: 284.4447. Conclusion: The increasing incidence of HIV infections, increasing incidence of organ transplantation, wide spread use of antibiotics and use of immunosuppressive agents are the important causative factors responsible for the increasing incidence of Candida infections. Furthermore, due to increasing resistance to various antifungal agents Candida has assumed greater clinical importance. This observation of the present study is in consonance with the changing trend seen both in India and across the world.https://ajms.alameenmedical.org/ArticlePDFs/8%20AJMS%20V18.N1.2025%20p%2042-46.pdfcandidaafstfluconazolenon-albicansinfection |
| spellingShingle | Paulami De Rehena Sarkar Raja Ray Kumkum Bhattacharyya Hasina Banu Hirak Jyoti Raj Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital Al Ameen Journal of Medical Sciences candida afst fluconazole non-albicans infection |
| title | Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital |
| title_full | Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital |
| title_fullStr | Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital |
| title_full_unstemmed | Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital |
| title_short | Study of antifungal susceptibility pattern of various Candida species with reference to conventional and automated methods in a tertiary care hospital |
| title_sort | study of antifungal susceptibility pattern of various candida species with reference to conventional and automated methods in a tertiary care hospital |
| topic | candida afst fluconazole non-albicans infection |
| url | https://ajms.alameenmedical.org/ArticlePDFs/8%20AJMS%20V18.N1.2025%20p%2042-46.pdf |
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