Detectability of cytokine and chemokine using ELISA, following sample-inactivation using Triton X-100 or heat

Detectability of cytokine and chemokine using ELISA, following sample-inactivation using Triton X-100 or heat

Abstract Clinical samples are routinely inactivated before molecular assays to prevent pathogen transmission. Antibody-based assays are sensitive to changes in analyte conformation, but the impact of inactivation on the analyte detectability has been overlooked. This study assessed the effects of co...

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Bibliographic Details
Main Authors: Erica Hofer Labossiere, Sandra Gonzalez-Diaz, Stephanie Enns, Paul Lopez, Xuefen Yang, Biniam Kidane, Gloria Vazquez-Grande, Abu Bakar Siddik, Sam Kam-Pun Kung, Paul Sandstrom, Amir Ravandi, T. Blake Ball, Ruey-Chyi Su
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Triton X-100
Cytokine/chemokine
Heat-inactivation
Lung aspirate
Nasopharyngeal swab
ELISA
Online Access:https://doi.org/10.1038/s41598-024-74739-0
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https://doi.org/10.1038/s41598-024-74739-0

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