Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii
Colistin (CT) is the last-resort of antibiotic against multidrug-resistance (MDR) Acinetobacter baumannii (A. baumannii) infection. However, colistin resistance is increasingly reported in A. baumannii isolates partially due to the global emergence and dissemination of plasmid-borne mobile colistin...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468682/full |
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| author | Meisong Li Furong Ma Hui Zhao Dianrong Zhou Dianrong Zhou Dianrong Zhou Lujie Liang Lujie Liang Lujie Liang Runling Lv Jiachen Li Jiachen Li Jiachen Li Yaxuan Wang Yaxuan Wang Yaxuan Wang Lin Xu Lin Xu Lin Xu Chenfei Liu Guo-Bao Tian Guo-Bao Tian Guo-Bao Tian Siyuan Feng Siyuan Feng Siyuan Feng Yong Xia |
| author_facet | Meisong Li Furong Ma Hui Zhao Dianrong Zhou Dianrong Zhou Dianrong Zhou Lujie Liang Lujie Liang Lujie Liang Runling Lv Jiachen Li Jiachen Li Jiachen Li Yaxuan Wang Yaxuan Wang Yaxuan Wang Lin Xu Lin Xu Lin Xu Chenfei Liu Guo-Bao Tian Guo-Bao Tian Guo-Bao Tian Siyuan Feng Siyuan Feng Siyuan Feng Yong Xia |
| author_sort | Meisong Li |
| collection | DOAJ |
| description | Colistin (CT) is the last-resort of antibiotic against multidrug-resistance (MDR) Acinetobacter baumannii (A. baumannii) infection. However, colistin resistance is increasingly reported in A. baumannii isolates partially due to the global emergence and dissemination of plasmid-borne mobile colistin resistance (mcr) gene and is a threat to human health. Thus, available treatment strategies urgently required in the fight against colistin-resistant A. baumannii. Here, we showed that mcr confers damaged outer membrane (OM) permeability in A. baumannii, which could compromise the viability of A. baumannii. Consistently, A. baumannii with colistin resistance exhibits increased susceptibility to macromolecular antibiotics such as rifampicin (RIF) and erythromycin (ERY). Moreover, the combination therapy of colistin and rifampicin demonstrates efficacy against colistin-resistant A. baumannii, regardless of the presence of mcr. Altogether, our data suggest that the synergy of colistin in combination with macromolecular hydrophobic antibiotics poses a promising therapeutic alternative for colistin-resistant A. baumannii. |
| format | Article |
| id | doaj-art-268d6eb0d36c401aa49dd53b6f7b7866 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-268d6eb0d36c401aa49dd53b6f7b78662024-11-19T06:15:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14686821468682Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumanniiMeisong Li0Furong Ma1Hui Zhao2Dianrong Zhou3Dianrong Zhou4Dianrong Zhou5Lujie Liang6Lujie Liang7Lujie Liang8Runling Lv9Jiachen Li10Jiachen Li11Jiachen Li12Yaxuan Wang13Yaxuan Wang14Yaxuan Wang15Lin Xu16Lin Xu17Lin Xu18Chenfei Liu19Guo-Bao Tian20Guo-Bao Tian21Guo-Bao Tian22Siyuan Feng23Siyuan Feng24Siyuan Feng25Yong Xia26Department of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Center for Provincial Clinical Research Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Center for Provincial Clinical Research Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, ChinaSchool of Pharmacy, Guangzhou Xinhua University, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Center for Provincial Clinical Research Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Center for Provincial Clinical Research Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Immunology and Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaAdvanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaDepartment of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Center for Provincial Clinical Research Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, ChinaColistin (CT) is the last-resort of antibiotic against multidrug-resistance (MDR) Acinetobacter baumannii (A. baumannii) infection. However, colistin resistance is increasingly reported in A. baumannii isolates partially due to the global emergence and dissemination of plasmid-borne mobile colistin resistance (mcr) gene and is a threat to human health. Thus, available treatment strategies urgently required in the fight against colistin-resistant A. baumannii. Here, we showed that mcr confers damaged outer membrane (OM) permeability in A. baumannii, which could compromise the viability of A. baumannii. Consistently, A. baumannii with colistin resistance exhibits increased susceptibility to macromolecular antibiotics such as rifampicin (RIF) and erythromycin (ERY). Moreover, the combination therapy of colistin and rifampicin demonstrates efficacy against colistin-resistant A. baumannii, regardless of the presence of mcr. Altogether, our data suggest that the synergy of colistin in combination with macromolecular hydrophobic antibiotics poses a promising therapeutic alternative for colistin-resistant A. baumannii.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468682/fullAcinetobacter baumanniicolistinrifampicinMCRouter membrane permeability |
| spellingShingle | Meisong Li Furong Ma Hui Zhao Dianrong Zhou Dianrong Zhou Dianrong Zhou Lujie Liang Lujie Liang Lujie Liang Runling Lv Jiachen Li Jiachen Li Jiachen Li Yaxuan Wang Yaxuan Wang Yaxuan Wang Lin Xu Lin Xu Lin Xu Chenfei Liu Guo-Bao Tian Guo-Bao Tian Guo-Bao Tian Siyuan Feng Siyuan Feng Siyuan Feng Yong Xia Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii Frontiers in Microbiology Acinetobacter baumannii colistin rifampicin MCR outer membrane permeability |
| title | Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii |
| title_full | Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii |
| title_fullStr | Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii |
| title_full_unstemmed | Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii |
| title_short | Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistant Acinetobacter baumannii |
| title_sort | outer membrane permeability of mcr positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin resistant acinetobacter baumannii |
| topic | Acinetobacter baumannii colistin rifampicin MCR outer membrane permeability |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1468682/full |
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