Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast
Cancercells are defined by abnormal and unrestricted mitotic divisions, thereforetargeting mitosis is a useful strategy for cancer treatment. Two groups ofdrugs that are most successfully used in the treatment of several types ofcancer, taxanes and vincaalkaloids, exhibit their anti-tumor effects by...
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Sakarya University
2020-02-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/967522 |
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| author | Pinar Buket Atalay Nur Kaluc Elif Ergin Cavusoglu |
| author_facet | Pinar Buket Atalay Nur Kaluc Elif Ergin Cavusoglu |
| author_sort | Pinar Buket Atalay |
| collection | DOAJ |
| description | Cancercells are defined by abnormal and unrestricted mitotic divisions, thereforetargeting mitosis is a useful strategy for cancer treatment. Two groups ofdrugs that are most successfully used in the treatment of several types ofcancer, taxanes and vincaalkaloids, exhibit their anti-tumor effects by causing a mitotic arrest.However, not much is known about whether being arrested in mitosis affects thesensitivity of cells to tumor-related stresses, such as oxidative and osmoticstresses. In this study, we investigated whether mitosis affects thesensitivity of Saccharomyces cerevisiaecells to H2O2-induced oxidative stress andsorbitol-induced osmotic stress. Mitotic and G1-arrests were induced bynocodazole and alpha factor, respectively. The effects of nocodazole or alphafactor treatments on the sensitivity of wild type (WT) and MAD3 deletion (mad3Δ) strains tooxidative stress and osmotic stress were evaluated by the spotting and coloniforming unit (cfu) assays as well as detection of reactive oxygen species (ROS)production. Data wereanalyzed using Student’s t-test and expressed as standard deviation (std), p<0.05 was considered significant. Our data indicate that mitosissignificantly increases resistance to oxidative stress, however itdoes not have any significant effect on the osmotic stress resistance in yeast. |
| format | Article |
| id | doaj-art-2623d9de1b564c2b9840a4fa230ca290 |
| institution | Kabale University |
| issn | 2147-835X |
| language | English |
| publishDate | 2020-02-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-2623d9de1b564c2b9840a4fa230ca2902024-12-23T08:05:05ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2020-02-0124126527110.16984/saufenbilder.55982028Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in YeastPinar Buket Atalay0https://orcid.org/0000-0001-7627-0291Nur Kaluc1https://orcid.org/0000-0002-7323-3150Elif Ergin Cavusoglu2https://orcid.org/0000-0002-0573-6047FACULTY OF MEDICINEFACULTY OF MEDICINEMarmara University Institute of Health SciencesCancercells are defined by abnormal and unrestricted mitotic divisions, thereforetargeting mitosis is a useful strategy for cancer treatment. Two groups ofdrugs that are most successfully used in the treatment of several types ofcancer, taxanes and vincaalkaloids, exhibit their anti-tumor effects by causing a mitotic arrest.However, not much is known about whether being arrested in mitosis affects thesensitivity of cells to tumor-related stresses, such as oxidative and osmoticstresses. In this study, we investigated whether mitosis affects thesensitivity of Saccharomyces cerevisiaecells to H2O2-induced oxidative stress andsorbitol-induced osmotic stress. Mitotic and G1-arrests were induced bynocodazole and alpha factor, respectively. The effects of nocodazole or alphafactor treatments on the sensitivity of wild type (WT) and MAD3 deletion (mad3Δ) strains tooxidative stress and osmotic stress were evaluated by the spotting and coloniforming unit (cfu) assays as well as detection of reactive oxygen species (ROS)production. Data wereanalyzed using Student’s t-test and expressed as standard deviation (std), p<0.05 was considered significant. Our data indicate that mitosissignificantly increases resistance to oxidative stress, however itdoes not have any significant effect on the osmotic stress resistance in yeast.https://dergipark.org.tr/tr/download/article-file/967522oxidative stressosmotic stressmitosissaccharomyces cerevisiae |
| spellingShingle | Pinar Buket Atalay Nur Kaluc Elif Ergin Cavusoglu Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi oxidative stress osmotic stress mitosis saccharomyces cerevisiae |
| title | Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast |
| title_full | Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast |
| title_fullStr | Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast |
| title_full_unstemmed | Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast |
| title_short | Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast |
| title_sort | effect of mitosis on the resistance to oxidative and osmotic stresses in yeast |
| topic | oxidative stress osmotic stress mitosis saccharomyces cerevisiae |
| url | https://dergipark.org.tr/tr/download/article-file/967522 |
| work_keys_str_mv | AT pinarbuketatalay effectofmitosisontheresistancetooxidativeandosmoticstressesinyeast AT nurkaluc effectofmitosisontheresistancetooxidativeandosmoticstressesinyeast AT elifergincavusoglu effectofmitosisontheresistancetooxidativeandosmoticstressesinyeast |