Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells
Three novel acidic polysaccharide fractions (OFPP-1, OFPP-2, OFPP-3) with different m olecular weights (803.7, 555.1 and 414.5 kDa) were isolated from the peeled Opuntia dillenii Haw. fruits by alkali-extraction, graded alcohol precipitation and column chromatography. Structural analysis indicated t...
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250160 |
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| author | Rui Liu Fangxin Chu Zheng Yan Hanqing Chen |
| author_facet | Rui Liu Fangxin Chu Zheng Yan Hanqing Chen |
| author_sort | Rui Liu |
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| description | Three novel acidic polysaccharide fractions (OFPP-1, OFPP-2, OFPP-3) with different m olecular weights (803.7, 555.1 and 414.5 kDa) were isolated from the peeled Opuntia dillenii Haw. fruits by alkali-extraction, graded alcohol precipitation and column chromatography. Structural analysis indicated that OFPPs were pectic polysaccharides consisting of rhamnose, arabinose and galactose residues. The backbone of OFPP-1 consisted of a repeating unit →6-α-D-GalpA-(1→2)-α-L-Rhap-(1→ with T-α-D-GalpA-(1→6)-α-D-GalpA-(1→4)-α-D-Glcp-(1→, T-β-D-Xylp-(1→6)-α-D-GalpA-(1→4)-α-D-Glcp-(1→ or T-α-D-GalpA-(1→3)-α-L-Araf-(1→as the side chains. The backbone of OFPP-2 consisted of a disaccharide repeating unit →2)-α-L-Rhap-(1→4)-β-D-GalpA-(1→ with T-β-L-Araf-(1→ as the branches substituted at the O-4 position of →2,4)-α-L-Rhap-(1→. Whereas the backbone of OFPP-3 was →2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→3)-β-L-Araf-(1→or →2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→4)-β-D-GalpA-(1→, which was branched at the O-4 position of→2,4)-α-L-Rhap-(1→. Moreover, these three polysaccharide fractions could protect Huh -7 cells against H2O2-induced oxidative stress to different extents by decreasing the MDA content and increasing the SOD, CAT, GSH-Px activities and the GSH level in the Huh-7 cells. These results suggest that OFPPs have the potential to be used as natural antioxidants. |
| format | Article |
| id | doaj-art-25cfdf1ae5864cc28ec4f67aeb4fd171 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-25cfdf1ae5864cc28ec4f67aeb4fd1712025-01-10T06:56:47ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011341929194210.26599/FSHW.2022.9250160Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cellsRui Liu0Fangxin Chu1Zheng Yan2Hanqing Chen3School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaThree novel acidic polysaccharide fractions (OFPP-1, OFPP-2, OFPP-3) with different m olecular weights (803.7, 555.1 and 414.5 kDa) were isolated from the peeled Opuntia dillenii Haw. fruits by alkali-extraction, graded alcohol precipitation and column chromatography. Structural analysis indicated that OFPPs were pectic polysaccharides consisting of rhamnose, arabinose and galactose residues. The backbone of OFPP-1 consisted of a repeating unit →6-α-D-GalpA-(1→2)-α-L-Rhap-(1→ with T-α-D-GalpA-(1→6)-α-D-GalpA-(1→4)-α-D-Glcp-(1→, T-β-D-Xylp-(1→6)-α-D-GalpA-(1→4)-α-D-Glcp-(1→ or T-α-D-GalpA-(1→3)-α-L-Araf-(1→as the side chains. The backbone of OFPP-2 consisted of a disaccharide repeating unit →2)-α-L-Rhap-(1→4)-β-D-GalpA-(1→ with T-β-L-Araf-(1→ as the branches substituted at the O-4 position of →2,4)-α-L-Rhap-(1→. Whereas the backbone of OFPP-3 was →2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→3)-β-L-Araf-(1→or →2,4)-α-L-Rhap-(1→2)-α-L-Rhap-(1→4)-β-D-GalpA-(1→, which was branched at the O-4 position of→2,4)-α-L-Rhap-(1→. Moreover, these three polysaccharide fractions could protect Huh -7 cells against H2O2-induced oxidative stress to different extents by decreasing the MDA content and increasing the SOD, CAT, GSH-Px activities and the GSH level in the Huh-7 cells. These results suggest that OFPPs have the potential to be used as natural antioxidants.https://www.sciopen.com/article/10.26599/FSHW.2022.9250160opuntia dillenii haw. fruitspolysaccharidealkali extractionstructural characterizationoxidative stress |
| spellingShingle | Rui Liu Fangxin Chu Zheng Yan Hanqing Chen Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells Food Science and Human Wellness opuntia dillenii haw. fruits polysaccharide alkali extraction structural characterization oxidative stress |
| title | Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells |
| title_full | Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells |
| title_fullStr | Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells |
| title_full_unstemmed | Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells |
| title_short | Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells |
| title_sort | structural characterization of three acidic polysaccharides from opuntia dillenii haw fruits and their protective effect against hydrogen peroxide induced oxidative stress in huh 7 cells |
| topic | opuntia dillenii haw. fruits polysaccharide alkali extraction structural characterization oxidative stress |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250160 |
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