A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate

A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate

Abstract Although DNA extraction is a crucial step before polymerase chain reaction (PCR), attempts to perform PCR without DNA isolation have been ongoing since the 1990s. While partial success has been achieved with direct conventional PCR, the DNA isolation step has remained indispensable for real...

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Bibliographic Details
Main Authors: Gökçe Güllü Amuran, Büşra Polat, Abdülkadir Kahraman, Mustafa Akkiprik
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Direct real-time PCR
DNA isolation
Direct PCR
Real-time PCR
Method
Online Access:https://doi.org/10.1038/s41598-024-78802-8
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https://doi.org/10.1038/s41598-024-78802-8

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