Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation
This study sought to explore the value of major ozonated autohemotherapy (MOA) as a treatment for spinal cord injury (SCI) in a rat model system. In total, 54 female Sprague-Dawley rats were randomized into sham-operated, SCI model, and MOA treatment groups. We found that relative to the SCI model g...
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De Gruyter
2024-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-1004 |
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| author | Xia Liwei Sun Yongming Zhou Yue Yang Qian Huang Jianhan Liu Dong |
| author_facet | Xia Liwei Sun Yongming Zhou Yue Yang Qian Huang Jianhan Liu Dong |
| author_sort | Xia Liwei |
| collection | DOAJ |
| description | This study sought to explore the value of major ozonated autohemotherapy (MOA) as a treatment for spinal cord injury (SCI) in a rat model system. In total, 54 female Sprague-Dawley rats were randomized into sham-operated, SCI model, and MOA treatment groups. We found that relative to the SCI model group, rats that underwent MOA treatment exhibited improved locomotor scores on days 14, 21, and 28 after injury (p < 0.05) together with reduced residual urine on days 5, 7, 14, and 21 after injury (p < 0.05). MOA treatment also lowered proinflammatory TNF-α, IL-1α, and C1q levels on day 3 post-injury (p < 0.05), decreased malondialdehyde levels, and enhanced superoxide dismutase activity (p < 0.001). Activated astrocytes in MOA-treated rats exhibited larger soma and higher levels of extracellular matrix secretion, whereas reactive microglia in the MOA group presented with a ramified morphology in contrast to the amoeboid morphology exhibited by these cells in SCI model rats. MOA offers potential value as a means of protecting spinal cord integrity, potentially through anti-inflammatory, antioxidant, and regulatory effects that shape the polarization of astrocytes and microglia. |
| format | Article |
| id | doaj-art-24f30278c563487893ffae2ae780b788 |
| institution | Kabale University |
| issn | 2391-5412 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-24f30278c563487893ffae2ae780b7882025-01-07T07:55:32ZengDe GruyterOpen Life Sciences2391-54122024-12-011916546810.1515/biol-2022-1004Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammationXia Liwei0Sun Yongming1Zhou Yue2Yang Qian3Huang Jianhan4Liu Dong5Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, ChinaDepartment of Clinical Medicine, Suzhou Medical College of Soochow University, Suzhou, 215000, ChinaDepartment of Orthopaedics, Guangxi Zhuang Autonomous Region Jiangbin Hospital, Nanning, 532001, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, ChinaThis study sought to explore the value of major ozonated autohemotherapy (MOA) as a treatment for spinal cord injury (SCI) in a rat model system. In total, 54 female Sprague-Dawley rats were randomized into sham-operated, SCI model, and MOA treatment groups. We found that relative to the SCI model group, rats that underwent MOA treatment exhibited improved locomotor scores on days 14, 21, and 28 after injury (p < 0.05) together with reduced residual urine on days 5, 7, 14, and 21 after injury (p < 0.05). MOA treatment also lowered proinflammatory TNF-α, IL-1α, and C1q levels on day 3 post-injury (p < 0.05), decreased malondialdehyde levels, and enhanced superoxide dismutase activity (p < 0.001). Activated astrocytes in MOA-treated rats exhibited larger soma and higher levels of extracellular matrix secretion, whereas reactive microglia in the MOA group presented with a ramified morphology in contrast to the amoeboid morphology exhibited by these cells in SCI model rats. MOA offers potential value as a means of protecting spinal cord integrity, potentially through anti-inflammatory, antioxidant, and regulatory effects that shape the polarization of astrocytes and microglia.https://doi.org/10.1515/biol-2022-1004scimajor ozonated autohemotherapyinflammationsuperoxide dismutase |
| spellingShingle | Xia Liwei Sun Yongming Zhou Yue Yang Qian Huang Jianhan Liu Dong Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation Open Life Sciences sci major ozonated autohemotherapy inflammation superoxide dismutase |
| title | Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| title_full | Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| title_fullStr | Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| title_full_unstemmed | Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| title_short | Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| title_sort | major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation |
| topic | sci major ozonated autohemotherapy inflammation superoxide dismutase |
| url | https://doi.org/10.1515/biol-2022-1004 |
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