Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway
We evaluated the effect of isoquercetin (quercetin-O-3-glucoside-quercetin, IQ) as a functional component of Abeliophyllum disistichum Nakai ethanol extract (ADLE) on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia (BPH). M...
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250216 |
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| author | Young-Jin Choi Meiqi Fan Nishala Erandi Wedamulla Yujiao Tang Eun-Kyung Kim |
| author_facet | Young-Jin Choi Meiqi Fan Nishala Erandi Wedamulla Yujiao Tang Eun-Kyung Kim |
| author_sort | Young-Jin Choi |
| collection | DOAJ |
| description | We evaluated the effect of isoquercetin (quercetin-O-3-glucoside-quercetin, IQ) as a functional component of Abeliophyllum disistichum Nakai ethanol extract (ADLE) on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia (BPH). Metabolites in ADLE were analyzed using UHPLC-qTOF-MS and HPLC. IQ was orally administered (1 or 10 mg/kg) to a testosterone propionate-induced BPH rat model, and its effects on the prostate weight were evaluated. The effect of IQ on androgen receptor (AR) signaling was analyzed in LNCaP cells. Whether IGF-1 and IQ affect the IGF-1/PI3K/Akt/mTOR pathway in BPH-1 cells was also examined. The metabolites in ADLE were identified and quantified, which confirmed that ADLE contained abundant IQ (20.88 mg/g). IQ significantly reduced the prostate size in a concentration-dependent manner in a BPH rat model, and significantly decreased the expression of AR signaling factors in the rat prostate tissue and LNCaP cells in a concentration-dependent manner. IQ also inhibited the PI3K/AKT/mTOR pathway activated by IGF-1 treatment in BPH-1 cells. In BPH-1 cells, IQ led to G0/G1 arrest and suppressed the expression of proliferation factors while inducing apoptosis. Thus, IQ shows potential for use as a pharmaceutical and nutraceutical for BPH. |
| format | Article |
| id | doaj-art-24da4e3644174882844fe8fa635edcc7 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-24da4e3644174882844fe8fa635edcc72025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331698171010.26599/FSHW.2022.9250216Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathwayYoung-Jin Choi0Meiqi Fan1Nishala Erandi Wedamulla2Yujiao Tang3Eun-Kyung Kim4Department of Food Science and Nutrition, Dong-A University, Busan 49315, Republic of KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of KoreaDepartment of Food Science and Nutrition, Dong-A University, Busan 49315, Republic of KoreaSchool of Bio-Science and Food Engineering, Changchun University of Science and Technology, Changchun 130600, ChinaNutritional Education Major, Graduate School of Education, Dong-A University, Busan 49315, Republic of KoreaWe evaluated the effect of isoquercetin (quercetin-O-3-glucoside-quercetin, IQ) as a functional component of Abeliophyllum disistichum Nakai ethanol extract (ADLE) on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia (BPH). Metabolites in ADLE were analyzed using UHPLC-qTOF-MS and HPLC. IQ was orally administered (1 or 10 mg/kg) to a testosterone propionate-induced BPH rat model, and its effects on the prostate weight were evaluated. The effect of IQ on androgen receptor (AR) signaling was analyzed in LNCaP cells. Whether IGF-1 and IQ affect the IGF-1/PI3K/Akt/mTOR pathway in BPH-1 cells was also examined. The metabolites in ADLE were identified and quantified, which confirmed that ADLE contained abundant IQ (20.88 mg/g). IQ significantly reduced the prostate size in a concentration-dependent manner in a BPH rat model, and significantly decreased the expression of AR signaling factors in the rat prostate tissue and LNCaP cells in a concentration-dependent manner. IQ also inhibited the PI3K/AKT/mTOR pathway activated by IGF-1 treatment in BPH-1 cells. In BPH-1 cells, IQ led to G0/G1 arrest and suppressed the expression of proliferation factors while inducing apoptosis. Thus, IQ shows potential for use as a pharmaceutical and nutraceutical for BPH.https://www.sciopen.com/article/10.26599/FSHW.2022.9250216isoquercetinbenign prostatic hyperplasiaandrogen receptor signalingpi3k/akt/mtor pathway |
| spellingShingle | Young-Jin Choi Meiqi Fan Nishala Erandi Wedamulla Yujiao Tang Eun-Kyung Kim Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway Food Science and Human Wellness isoquercetin benign prostatic hyperplasia androgen receptor signaling pi3k/akt/mtor pathway |
| title | Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway |
| title_full | Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway |
| title_fullStr | Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway |
| title_full_unstemmed | Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway |
| title_short | Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway |
| title_sort | alleviatory effect of isoquercetin on benign prostatic hyperplasia via igf 1 pi3k akt mtor pathway |
| topic | isoquercetin benign prostatic hyperplasia androgen receptor signaling pi3k/akt/mtor pathway |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250216 |
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