Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing

Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing

Abstract Background Long-read 16S rRNA gene amplicon sequencing has a high potential for characterizing food-associated microbiomes. The advantage results from sequencing the full-length (1,500 bp) gene, enabling taxonomic resolution at species level. Here we present a benchmarking study using mock...

Full description

Saved in:
Bibliographic Details
Main Authors: Mareike Baer, Lisa Höppe, Waldemar Seel, André Lipski
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Microbiology
Subjects:
PacBio sequencing
Full-length 16S rRNA gene
Long-read sequencing
Amplicon sequencing
Mock community
Food microbiota
Online Access:https://doi.org/10.1186/s12866-024-03677-8
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doi.org/10.1186/s12866-024-03677-8

Similar Items