cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India
Background: Staphylococcus haemolyticus is a clinically significant Coagulase Negative Staphylococci (CoNS) accountable for various infections and is often multi-drug resistant along with resistance to linezolid. Aim and Objectives: This study was carried out to detect the presence of cfr and optrA...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Krishna Vishwa Vidyapeeth (Deemed to be University), Karad
2024-01-01
|
| Series: | Journal of Krishna Institute of Medical Sciences University |
| Subjects: | |
| Online Access: | https://jkimsu.com/jkimsu-vol13no1/JKIMSU,%20Vol.%2013,%20No.%201,%20January-March%202024%20Page%2058-66.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850160786971820032 |
|---|---|
| author | Km. Sangita Geeta Gupta Anita Pandey Ankita Gupta |
| author_facet | Km. Sangita Geeta Gupta Anita Pandey Ankita Gupta |
| author_sort | Km. Sangita |
| collection | DOAJ |
| description | Background: Staphylococcus haemolyticus is a clinically significant Coagulase Negative Staphylococci (CoNS) accountable for various infections and is often multi-drug resistant along with resistance to linezolid. Aim and Objectives: This study was carried out to detect the presence of cfr and optrA gene in clinical isolates of linezolid resistant S. haemolyticus in a tertiary care hospital. Material and Methods: A total of 227 clinical isolates of S. haemolyticus recovered from various clinical samples were tested to determine susceptibility to linezolid by
phenotypic method. Further, the presence of cfr & optrA gene was looked for, in clinical isolates of S. haemolyticus. Results: S. haemolyticus was isolated predominantly from inpatient department mainly from blood culture (85%).
Linezolid resistance was confirmed in 9.2% isolates of S. haemolyticus phenotypically and they were also methicillin resistant. On genotypic characterization, cfr gene could be detected in 7.04% the isolates. However, all the isolates were negative for optrA gene. Conclusion: Emergence of resistance to linezolid in S. haemolyticus was reported for the first time. The presence of cfr gene is the most common mechanism of resistance to linezolid. Correct
identification of these isolates and adherence to strict infection control protocols will help in better clinical outcomes. |
| format | Article |
| id | doaj-art-216260bf5e1e429f901b2e058c0ffd97 |
| institution | OA Journals |
| issn | 2231-4261 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Krishna Vishwa Vidyapeeth (Deemed to be University), Karad |
| record_format | Article |
| series | Journal of Krishna Institute of Medical Sciences University |
| spelling | doaj-art-216260bf5e1e429f901b2e058c0ffd972025-08-20T02:23:04ZengKrishna Vishwa Vidyapeeth (Deemed to be University), KaradJournal of Krishna Institute of Medical Sciences University2231-42612024-01-011315866cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, IndiaKm. Sangita0Geeta Gupta1Anita Pandey2Ankita Gupta3Department of Microbiology, Santosh Medical College & Hospital (Deemed to be University) Ghaziabad-201009 (Uttar Pradesh), India Department of Microbiology, Santosh Medical College & Hospital (Deemed to be University) Ghaziabad-201009 (Uttar Pradesh), India Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University Meerut-250002 (Uttar Pradesh), IndiaDepartment of Microbiology, Santosh Medical College & Hospital (Deemed to be University) Ghaziabad-201009 (Uttar Pradesh), India Background: Staphylococcus haemolyticus is a clinically significant Coagulase Negative Staphylococci (CoNS) accountable for various infections and is often multi-drug resistant along with resistance to linezolid. Aim and Objectives: This study was carried out to detect the presence of cfr and optrA gene in clinical isolates of linezolid resistant S. haemolyticus in a tertiary care hospital. Material and Methods: A total of 227 clinical isolates of S. haemolyticus recovered from various clinical samples were tested to determine susceptibility to linezolid by phenotypic method. Further, the presence of cfr & optrA gene was looked for, in clinical isolates of S. haemolyticus. Results: S. haemolyticus was isolated predominantly from inpatient department mainly from blood culture (85%). Linezolid resistance was confirmed in 9.2% isolates of S. haemolyticus phenotypically and they were also methicillin resistant. On genotypic characterization, cfr gene could be detected in 7.04% the isolates. However, all the isolates were negative for optrA gene. Conclusion: Emergence of resistance to linezolid in S. haemolyticus was reported for the first time. The presence of cfr gene is the most common mechanism of resistance to linezolid. Correct identification of these isolates and adherence to strict infection control protocols will help in better clinical outcomes.https://jkimsu.com/jkimsu-vol13no1/JKIMSU,%20Vol.%2013,%20No.%201,%20January-March%202024%20Page%2058-66.pdfcoagulase negative staphylococcistaphylococcus haemolyticuslinezolid resistancecfroptragene |
| spellingShingle | Km. Sangita Geeta Gupta Anita Pandey Ankita Gupta cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India Journal of Krishna Institute of Medical Sciences University coagulase negative staphylococci staphylococcus haemolyticus linezolid resistance cfr optragene |
| title | cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India |
| title_full | cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India |
| title_fullStr | cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India |
| title_full_unstemmed | cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India |
| title_short | cfr and optrA gene based characterization of linezolid resistant Staphylococcus haemolyticus in a tertiary care hospital in western Uttar Pradesh, India |
| title_sort | cfr and optra gene based characterization of linezolid resistant staphylococcus haemolyticus in a tertiary care hospital in western uttar pradesh india |
| topic | coagulase negative staphylococci staphylococcus haemolyticus linezolid resistance cfr optragene |
| url | https://jkimsu.com/jkimsu-vol13no1/JKIMSU,%20Vol.%2013,%20No.%201,%20January-March%202024%20Page%2058-66.pdf |
| work_keys_str_mv | AT kmsangita cfrandoptragenebasedcharacterizationoflinezolidresistantstaphylococcushaemolyticusinatertiarycarehospitalinwesternuttarpradeshindia AT geetagupta cfrandoptragenebasedcharacterizationoflinezolidresistantstaphylococcushaemolyticusinatertiarycarehospitalinwesternuttarpradeshindia AT anitapandey cfrandoptragenebasedcharacterizationoflinezolidresistantstaphylococcushaemolyticusinatertiarycarehospitalinwesternuttarpradeshindia AT ankitagupta cfrandoptragenebasedcharacterizationoflinezolidresistantstaphylococcushaemolyticusinatertiarycarehospitalinwesternuttarpradeshindia |