Selection and evaluation of new reference genes for qRT-PCR analysis in humpback grouper Cromileptes altivelis based on transcriptome data

Selection and evaluation of new reference genes for qRT-PCR analysis in humpback grouper Cromileptes altivelis based on transcriptome data

Quantitative Real-Time PCR (qRT-PCR) is widely used in molecular biology research. A stable reference gene is essential for accurate quantification of target gene expression. Traditionally, these reference genes were selected from housekeeping genes. RNA-seq has enabled high-throughput selection. Th...

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Bibliographic Details
Main Authors: Xiaona Xu, Dailiang Kou, Huibang Sun, Chenxi Wang, Dongxue Xia, Zhennian Chen, Jinxiang Liu
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Aquaculture Reports
Subjects:
Reference genes
Transcriptome
Cromileptes altivelis
Gene expression
Stability and normalization
Online Access:http://www.sciencedirect.com/science/article/pii/S235251342500167X
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