Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria
Abstract Objective The aim of this study was to investigate the distribution and genetic determinants of carbapenemase production and colistin resistance among Acinetobacter baumannii isolates recovered from three health care facilities in the city of Batna, Algeria. Methods A prospective study was...
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BMC
2025-07-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-025-11198-6 |
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| author | Asma Bouali Esma Bendjama Zineb Cherak Meriem Mennaai Ahmed Kassah-Laouar Jean-Marc Rolain Lotfi Loucif |
| author_facet | Asma Bouali Esma Bendjama Zineb Cherak Meriem Mennaai Ahmed Kassah-Laouar Jean-Marc Rolain Lotfi Loucif |
| author_sort | Asma Bouali |
| collection | DOAJ |
| description | Abstract Objective The aim of this study was to investigate the distribution and genetic determinants of carbapenemase production and colistin resistance among Acinetobacter baumannii isolates recovered from three health care facilities in the city of Batna, Algeria. Methods A prospective study was conducted between 2021 and 2022 on 46 Acinetobacter baumannii clinical isolates, which were collected and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility testing was performed using the disk diffusion method and colistin minimum inhibitory concentrations (MICs) were determined by broth microdilution method. Carbapenemase and colistin resistance determinants were detected by qPCR. Results The 46 clinical isolates were mainly from the intensive care unit (52.17%) and the burns unit (17.39%). The strains were collected primarily from pus samples (34.78%) and blood samples (17.39%). Eleven strains were classified as colistin-resistant, with MICs ranging from 4 to 128 μg/mL. The bla OXA-24 gene was detected in 63.04% of the isolates, followed by the bla OXA-23 gene (43.47%). Nine strains were positive for both bla OXA-23-like and bla OXA-24-like genes. The bla NDM gene was detected in eight isolates (17.39%), including two which co-expressed a bla OXA-24 gene. In contrast, all strains were negative for the plasmid-mediated colistin resistance mcr-1 to mcr-5 and mcr-8. Conclusion Here, we report a high prevalence of carbapenemases-producing A. baumannii isolates in Batna hospitals. Notably, this study is the first to identify A. baumannii isolates co-producing OXA-24 and NDM carbapenemases and to report the first detection of colistin-resistant A. baumannii co-producing OXA-24 and OXA-23 carbapenemases from a patient in Algeria. |
| format | Article |
| id | doaj-art-1f4b2de8aec74d16b82a2ba6c7bf0a5a |
| institution | Kabale University |
| issn | 1471-2334 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj-art-1f4b2de8aec74d16b82a2ba6c7bf0a5a2025-08-20T03:45:24ZengBMCBMC Infectious Diseases1471-23342025-07-012511910.1186/s12879-025-11198-6Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, AlgeriaAsma Bouali0Esma Bendjama1Zineb Cherak2Meriem Mennaai3Ahmed Kassah-Laouar4Jean-Marc Rolain5Lotfi Loucif6Laboratoire des Sciences Alimentaires, Institut des Sciences Vétérinaires et des Sciences Agronomiques, Université Batna 1Département de Technologie Alimentaire, Institut des Sciences Vétérinaires et des Sciences Agronomiques, Université Batna 1Département de Microbiologie et de Biochimie, Faculté des Sciences de la Nature et de la Vie, Université Batna 2Laboratoire Central de Biologie Médicale, Centre de Lutte Contre le CancerCentre de Diagnostic Médical El MoustakbelIHU Méditerranée Infection, MEPHI, Aix Marseille Université, Faculté de Médecine et de PharmacieDépartement de Microbiologie et de Biochimie, Faculté des Sciences de la Nature et de la Vie, Université Batna 2Abstract Objective The aim of this study was to investigate the distribution and genetic determinants of carbapenemase production and colistin resistance among Acinetobacter baumannii isolates recovered from three health care facilities in the city of Batna, Algeria. Methods A prospective study was conducted between 2021 and 2022 on 46 Acinetobacter baumannii clinical isolates, which were collected and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility testing was performed using the disk diffusion method and colistin minimum inhibitory concentrations (MICs) were determined by broth microdilution method. Carbapenemase and colistin resistance determinants were detected by qPCR. Results The 46 clinical isolates were mainly from the intensive care unit (52.17%) and the burns unit (17.39%). The strains were collected primarily from pus samples (34.78%) and blood samples (17.39%). Eleven strains were classified as colistin-resistant, with MICs ranging from 4 to 128 μg/mL. The bla OXA-24 gene was detected in 63.04% of the isolates, followed by the bla OXA-23 gene (43.47%). Nine strains were positive for both bla OXA-23-like and bla OXA-24-like genes. The bla NDM gene was detected in eight isolates (17.39%), including two which co-expressed a bla OXA-24 gene. In contrast, all strains were negative for the plasmid-mediated colistin resistance mcr-1 to mcr-5 and mcr-8. Conclusion Here, we report a high prevalence of carbapenemases-producing A. baumannii isolates in Batna hospitals. Notably, this study is the first to identify A. baumannii isolates co-producing OXA-24 and NDM carbapenemases and to report the first detection of colistin-resistant A. baumannii co-producing OXA-24 and OXA-23 carbapenemases from a patient in Algeria.https://doi.org/10.1186/s12879-025-11198-6Acinetobacter baumanniibla OXA-23bla OXA-24bla NDMAlgeria |
| spellingShingle | Asma Bouali Esma Bendjama Zineb Cherak Meriem Mennaai Ahmed Kassah-Laouar Jean-Marc Rolain Lotfi Loucif Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria BMC Infectious Diseases Acinetobacter baumannii bla OXA-23 bla OXA-24 bla NDM Algeria |
| title | Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria |
| title_full | Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria |
| title_fullStr | Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria |
| title_full_unstemmed | Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria |
| title_short | Distribution of carbapenemase-producing and colistin resistant Acinetobacter baumannii isolates in Batna hospitals, Algeria |
| title_sort | distribution of carbapenemase producing and colistin resistant acinetobacter baumannii isolates in batna hospitals algeria |
| topic | Acinetobacter baumannii bla OXA-23 bla OXA-24 bla NDM Algeria |
| url | https://doi.org/10.1186/s12879-025-11198-6 |
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