Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors
In this study 80 malignant CMT were submitted to immunohistochemical detection of CD3, c-kit, VEGF, and CD31, together with clinicopathological parameters of tumor aggressiveness. CD3+ T-cells and c-kit overexpression revealed a positive correlation with VEGF (r = 0.503, P < 0.0001; r = 0.284, P...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2015/920409 |
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| author | Maria Isabel Carvalho Isabel Pires Marlene Dias Justina Prada Hugo Gregório Luis Lobo Felisbina Queiroga |
| author_facet | Maria Isabel Carvalho Isabel Pires Marlene Dias Justina Prada Hugo Gregório Luis Lobo Felisbina Queiroga |
| author_sort | Maria Isabel Carvalho |
| collection | DOAJ |
| description | In this study 80 malignant CMT were submitted to immunohistochemical detection of CD3, c-kit, VEGF, and CD31, together with clinicopathological parameters of tumor aggressiveness. CD3+ T-cells and c-kit overexpression revealed a positive correlation with VEGF (r = 0.503, P < 0.0001; r = 0.284, P = 0.023 for CD3 and c-kit, resp.) and CD31 (r = 0.654, P < 0.0001; r = 0.365, P = 0.003 for CD3 and c-kit, resp.). A significant association (P = 0.039) and a positive correlation (r = 0.263, P = 0.039) between CD3 and c-kit were also observed. High CD3/VEGF, c-kit/VEGF, and CD3/c-kit tumors were associated with elevated grade of malignancy (P < 0.0001 for all groups), presence of intravascular emboli (P < 0.0001 for CD3/VEGF and CD3/c-kit; P = 0.002 for c-kit/VEGF), and presence of lymph node metastasis (P < 0.0001 for all groups). Tumors with high CD3/VEGF (P = 0.006), c-kit/VEGF (P < 0.0001), and CD3/c-kit (P = 0.002) were associated with poor prognosis. Interestingly high c-kit/VEGF tumors retained their significance by multivariate analysis arising as independent prognostic factor. |
| format | Article |
| id | doaj-art-1f0d56a0e8db4f72a7bf8acc9d9cd418 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-1f0d56a0e8db4f72a7bf8acc9d9cd4182025-08-20T03:54:34ZengWileyAnalytical Cellular Pathology2210-71772210-71852015-01-01201510.1155/2015/920409920409Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary TumorsMaria Isabel Carvalho0Isabel Pires1Marlene Dias2Justina Prada3Hugo Gregório4Luis Lobo5Felisbina Queiroga6CECAV, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalCECAV, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalSchool of Life Sciences and the Environment, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalCECAV, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalDepartment of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalHospital Veterinário do Porto, Travessa Silva Porto 174, 4250-475 Porto, PortugalDepartment of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, PortugalIn this study 80 malignant CMT were submitted to immunohistochemical detection of CD3, c-kit, VEGF, and CD31, together with clinicopathological parameters of tumor aggressiveness. CD3+ T-cells and c-kit overexpression revealed a positive correlation with VEGF (r = 0.503, P < 0.0001; r = 0.284, P = 0.023 for CD3 and c-kit, resp.) and CD31 (r = 0.654, P < 0.0001; r = 0.365, P = 0.003 for CD3 and c-kit, resp.). A significant association (P = 0.039) and a positive correlation (r = 0.263, P = 0.039) between CD3 and c-kit were also observed. High CD3/VEGF, c-kit/VEGF, and CD3/c-kit tumors were associated with elevated grade of malignancy (P < 0.0001 for all groups), presence of intravascular emboli (P < 0.0001 for CD3/VEGF and CD3/c-kit; P = 0.002 for c-kit/VEGF), and presence of lymph node metastasis (P < 0.0001 for all groups). Tumors with high CD3/VEGF (P = 0.006), c-kit/VEGF (P < 0.0001), and CD3/c-kit (P = 0.002) were associated with poor prognosis. Interestingly high c-kit/VEGF tumors retained their significance by multivariate analysis arising as independent prognostic factor.http://dx.doi.org/10.1155/2015/920409 |
| spellingShingle | Maria Isabel Carvalho Isabel Pires Marlene Dias Justina Prada Hugo Gregório Luis Lobo Felisbina Queiroga Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors Analytical Cellular Pathology |
| title | Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors |
| title_full | Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors |
| title_fullStr | Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors |
| title_full_unstemmed | Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors |
| title_short | Intratumoral CD3+ T-Lymphocytes Immunoexpression and Its Association with c-Kit, Angiogenesis, and Overall Survival in Malignant Canine Mammary Tumors |
| title_sort | intratumoral cd3 t lymphocytes immunoexpression and its association with c kit angiogenesis and overall survival in malignant canine mammary tumors |
| url | http://dx.doi.org/10.1155/2015/920409 |
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