Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival
Abstract Objectives Langerhans cells (LCs) are epithelial antigen‐presenting cells (APC) contributing to immune surveillance. LCs depend on interleukin 34 (IL34) production by epithelial cells. This study aimed to uncover mechanisms of alteration of IL34 and LC function in squamous cell carcinoma (S...
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| Format: | Article |
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Wiley
2024-01-01
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| Series: | Clinical & Translational Immunology |
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| Online Access: | https://doi.org/10.1002/cti2.70018 |
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| author | Thi Viet Trinh Dang Kevin R Gillinder Quan Nguyen Onkar Mulay Tuan Vo Ahmed M Mehdi Chenhao Zhou Andrew J Brooks Graham R Leggatt David A Hume Ian H Frazer Janin Chandra |
| author_facet | Thi Viet Trinh Dang Kevin R Gillinder Quan Nguyen Onkar Mulay Tuan Vo Ahmed M Mehdi Chenhao Zhou Andrew J Brooks Graham R Leggatt David A Hume Ian H Frazer Janin Chandra |
| author_sort | Thi Viet Trinh Dang |
| collection | DOAJ |
| description | Abstract Objectives Langerhans cells (LCs) are epithelial antigen‐presenting cells (APC) contributing to immune surveillance. LCs depend on interleukin 34 (IL34) production by epithelial cells. This study aimed to uncover mechanisms of alteration of IL34 and LC function in squamous cell carcinoma (SCC). Methods Cancer cohort data were used to identify associations between SCC and IL34. ATAC‐seq of keratinocytes (KCs) and LCs from a murine model of epithelial hyperplasia, driven by HPV16 E7 oncoprotein (K14E7), was analysed. Transcriptomic data were used to validate findings. RNAscope, RT‐qPCR, ELISA and confocal imaging was used to analyse IL34 expression and LCs in a spatial context. Results IL34 mRNA is downregulated in human SCCs of the head and neck, the cervix, the lung and the oesophagus, and low IL34 expression is associated with poor survival. We demonstrate that KCs of K14E7 mice have reduced Il34 gene accessibility, mRNA and protein, as well as broad changes in promotor accessibility associated with cell adhesion and immune responses. Chromatin accessibility was substantially changed in LCs, including increased accessibility of the Csf1r gene, and changes in promotors associated with cytoskeleton arrangement and antigen processing and presentation. We discovered altered spatial LC dendrite organisation in hyperproliferative epithelium. Conclusion Squamous cell carcinoma of the cervix, head and neck, oesophagus and lung demonstrate downregulation of IL34, which is associated with poor survival, and with alterations in LC spatial organisation and function. These findings suggest that reduced IL34 expression in SCC may contribute to impaired local immunity through LC dysregulation. |
| format | Article |
| id | doaj-art-1d915e0cfb554d64a5cd4447a9c4f024 |
| institution | Kabale University |
| issn | 2050-0068 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical & Translational Immunology |
| spelling | doaj-art-1d915e0cfb554d64a5cd4447a9c4f0242024-12-27T06:26:39ZengWileyClinical & Translational Immunology2050-00682024-01-011312n/an/a10.1002/cti2.70018Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survivalThi Viet Trinh Dang0Kevin R Gillinder1Quan Nguyen2Onkar Mulay3Tuan Vo4Ahmed M Mehdi5Chenhao Zhou6Andrew J Brooks7Graham R Leggatt8David A Hume9Ian H Frazer10Janin Chandra11Faculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaInstitute for Molecular Bioscience The University of Queensland Brisbane QLD AustraliaInstitute for Molecular Bioscience The University of Queensland Brisbane QLD AustraliaInstitute for Molecular Bioscience The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaMater Research Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaFaculty of Medicine, Frazer Institute The University of Queensland Brisbane QLD AustraliaAbstract Objectives Langerhans cells (LCs) are epithelial antigen‐presenting cells (APC) contributing to immune surveillance. LCs depend on interleukin 34 (IL34) production by epithelial cells. This study aimed to uncover mechanisms of alteration of IL34 and LC function in squamous cell carcinoma (SCC). Methods Cancer cohort data were used to identify associations between SCC and IL34. ATAC‐seq of keratinocytes (KCs) and LCs from a murine model of epithelial hyperplasia, driven by HPV16 E7 oncoprotein (K14E7), was analysed. Transcriptomic data were used to validate findings. RNAscope, RT‐qPCR, ELISA and confocal imaging was used to analyse IL34 expression and LCs in a spatial context. Results IL34 mRNA is downregulated in human SCCs of the head and neck, the cervix, the lung and the oesophagus, and low IL34 expression is associated with poor survival. We demonstrate that KCs of K14E7 mice have reduced Il34 gene accessibility, mRNA and protein, as well as broad changes in promotor accessibility associated with cell adhesion and immune responses. Chromatin accessibility was substantially changed in LCs, including increased accessibility of the Csf1r gene, and changes in promotors associated with cytoskeleton arrangement and antigen processing and presentation. We discovered altered spatial LC dendrite organisation in hyperproliferative epithelium. Conclusion Squamous cell carcinoma of the cervix, head and neck, oesophagus and lung demonstrate downregulation of IL34, which is associated with poor survival, and with alterations in LC spatial organisation and function. These findings suggest that reduced IL34 expression in SCC may contribute to impaired local immunity through LC dysregulation.https://doi.org/10.1002/cti2.70018epithelial hyperplasiaIL34Langerhans cellssquamous cell carcinoma |
| spellingShingle | Thi Viet Trinh Dang Kevin R Gillinder Quan Nguyen Onkar Mulay Tuan Vo Ahmed M Mehdi Chenhao Zhou Andrew J Brooks Graham R Leggatt David A Hume Ian H Frazer Janin Chandra Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival Clinical & Translational Immunology epithelial hyperplasia IL34 Langerhans cells squamous cell carcinoma |
| title | Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival |
| title_full | Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival |
| title_fullStr | Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival |
| title_full_unstemmed | Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival |
| title_short | Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen‐processing‐presentation machinery and poor patient survival |
| title_sort | squamous cell carcinoma is associated with reduced il34 expression alterations in the langerhans cell antigen processing presentation machinery and poor patient survival |
| topic | epithelial hyperplasia IL34 Langerhans cells squamous cell carcinoma |
| url | https://doi.org/10.1002/cti2.70018 |
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