One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity

Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging to establish an efficient RCA detection method. The false positive signals are primarily caused by binding of non-target nucleic acids to the circula...

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Bibliographic Details
Main Authors: Wenhua Sun, Kunling Hu, Ziting Song, Ran An, Xingguo Liang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Biosensors
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Online Access:https://www.mdpi.com/2079-6374/15/5/317
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