A High-Throughput Neurosphere-Based Colony Formation Assay to Test Drug and Radiation Sensitivity of Different Patient-Derived Glioblastoma Lines

A High-Throughput Neurosphere-Based Colony Formation Assay to Test Drug and Radiation Sensitivity of Different Patient-Derived Glioblastoma Lines

The gold standard assay for radiation response is the clonogenic assay, a normalized colony formation assay (CFA) that can capture a broad range of radiation-induced cell death mechanisms. Traditionally, this assay relies on two-dimensional (2D) cell culture conditions with colonies counted by fixin...

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Bibliographic Details
Main Authors: Manoj Kumar, Lauren C. Nassour-Caswell, Hasan Alrefai, Joshua C. Anderson, Taylor L. Schanel, Patricia H. Hicks, Rex Cardan, Christopher D. Willey
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Cells
Subjects:
colony formation assay
clonogenic assay
radiation sensitivity
glioblastoma
patient-derived cancer cells
brain tumor initiating cells
Online Access:https://www.mdpi.com/2073-4409/13/23/1995
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https://www.mdpi.com/2073-4409/13/23/1995

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