Splice-site mutation enhances the efficiency of CRISPR-mediated gene knockout and new germplasm creation in fish

Splice-site mutation enhances the efficiency of CRISPR-mediated gene knockout and new germplasm creation in fish

The knockout efficiency of the CRISPR/Cas9 system largely relies on the frameshift mutation caused by random insertions or deletions at target site of coding DNA sequence (CDS). Splice site (SS) mutations result in mRNA mis-splicing, making gRNAs targeting SS more likely to produce null mutations an...

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Bibliographic Details
Main Authors: Zhen Xu, Ma Zhuo, Leihui Wang, Shuo Yan, Shiyi Zhang, Ying Chen, Guang Xu, Chenxu Wang, Wenjing Tao, Deshou Wang
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
Subjects:
CRISPR/Cas9
gRNA targeting splice site
Exon skipping
Intron retention
Null mutation and phenotype rate
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425003813
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