Plant-based biomaterials as scaffolds for cellular agriculture
Edible scaffolds are the essential components for cultivated meat. This research aimed to evaluate the performance of food-grade polysaccharides (pectin and alginate) and proteins (soy protein isolate (SPI) and pea protein isolate (PPI)) as scaffolds for cultivated meat production. A myoblast model...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833524001679 |
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| author | Woo-Ju Kim Yoonbin Kim Reza Ovissipour Nitin Nitin |
| author_facet | Woo-Ju Kim Yoonbin Kim Reza Ovissipour Nitin Nitin |
| author_sort | Woo-Ju Kim |
| collection | DOAJ |
| description | Edible scaffolds are the essential components for cultivated meat. This research aimed to evaluate the performance of food-grade polysaccharides (pectin and alginate) and proteins (soy protein isolate (SPI) and pea protein isolate (PPI)) as scaffolds for cultivated meat production. A myoblast model cell line (C2C12) and an embryonic-derived fish cell line (ZEM2S) were selected as model cell lines. Rheological analysis revealed that the selected gels were viscoelastic solids with shear thinning behavior. The storage modulus (G') and loss modulus (G'') of pectin and PPI were greater than 1000 Pa and 100 Pa. The pectin gels exhibit better structure recovery compared to other biopolymer gels. The texture properties were similar to those of commercial meat analogues. Furthermore, the pectin gels were stable in water. In general, the cytocompatibility of the biomaterial gels was similar for the tested cell lines, except for ZEM2S when exposed to protein gels. Based on the results of mechanical properties and cytocompatibilities of gels, a 3D printed structure with pectin gel was generated. 3D-printed scaffolds promoted the proliferation of C2C12 cells during 5 days of incubation. These findings highlight the potential of plant biomaterials and 3D printing to develop scaffolds for the production of cultivated meat. |
| format | Article |
| id | doaj-art-19a407a18f744186a17e3d6805fc7b67 |
| institution | Kabale University |
| issn | 2666-8335 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj-art-19a407a18f744186a17e3d6805fc7b672024-12-18T08:53:48ZengElsevierFuture Foods2666-83352024-12-0110100468Plant-based biomaterials as scaffolds for cellular agricultureWoo-Ju Kim0Yoonbin Kim1Reza Ovissipour2Nitin Nitin3Department of Food Science and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea; Research Institute of Food and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USADepartment of Food Science and Technology, University of California-Davis, Davis, CA 95616, USADepartment of Food Science and Technology, Texas A&M University, College Station, Texas 77843, USADepartment of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA; Department of Biological and Agricultural Engineering, University of California-Davis, Davis, CA, 95616, USA; Corresponding author at: Department of Food Science and Technology, University of California-Davis, Davis, California 95616, USA.Edible scaffolds are the essential components for cultivated meat. This research aimed to evaluate the performance of food-grade polysaccharides (pectin and alginate) and proteins (soy protein isolate (SPI) and pea protein isolate (PPI)) as scaffolds for cultivated meat production. A myoblast model cell line (C2C12) and an embryonic-derived fish cell line (ZEM2S) were selected as model cell lines. Rheological analysis revealed that the selected gels were viscoelastic solids with shear thinning behavior. The storage modulus (G') and loss modulus (G'') of pectin and PPI were greater than 1000 Pa and 100 Pa. The pectin gels exhibit better structure recovery compared to other biopolymer gels. The texture properties were similar to those of commercial meat analogues. Furthermore, the pectin gels were stable in water. In general, the cytocompatibility of the biomaterial gels was similar for the tested cell lines, except for ZEM2S when exposed to protein gels. Based on the results of mechanical properties and cytocompatibilities of gels, a 3D printed structure with pectin gel was generated. 3D-printed scaffolds promoted the proliferation of C2C12 cells during 5 days of incubation. These findings highlight the potential of plant biomaterials and 3D printing to develop scaffolds for the production of cultivated meat.http://www.sciencedirect.com/science/article/pii/S2666833524001679Cultivated meatCultured meatScaffoldAlternative meat3D printing |
| spellingShingle | Woo-Ju Kim Yoonbin Kim Reza Ovissipour Nitin Nitin Plant-based biomaterials as scaffolds for cellular agriculture Future Foods Cultivated meat Cultured meat Scaffold Alternative meat 3D printing |
| title | Plant-based biomaterials as scaffolds for cellular agriculture |
| title_full | Plant-based biomaterials as scaffolds for cellular agriculture |
| title_fullStr | Plant-based biomaterials as scaffolds for cellular agriculture |
| title_full_unstemmed | Plant-based biomaterials as scaffolds for cellular agriculture |
| title_short | Plant-based biomaterials as scaffolds for cellular agriculture |
| title_sort | plant based biomaterials as scaffolds for cellular agriculture |
| topic | Cultivated meat Cultured meat Scaffold Alternative meat 3D printing |
| url | http://www.sciencedirect.com/science/article/pii/S2666833524001679 |
| work_keys_str_mv | AT woojukim plantbasedbiomaterialsasscaffoldsforcellularagriculture AT yoonbinkim plantbasedbiomaterialsasscaffoldsforcellularagriculture AT rezaovissipour plantbasedbiomaterialsasscaffoldsforcellularagriculture AT nitinnitin plantbasedbiomaterialsasscaffoldsforcellularagriculture |