Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment
Small cell carcinoma of the esophagus (SCCE) is a rare and aggressively progressing malignancy that presents considerable clinical challenges.Although chemotherapy can effectively manage symptoms during the earlystages of SCCE, its long-term effectiveness is notably limited, with theunderlying mecha...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1477705/full |
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| author | Qinkai Zhang Qinkai Zhang Qinkai Zhang Ziyu Gao Ru Qiu Jizhao Cao Chunxiao Zhang Wei Qin Wei Qin Meiling Yang Meiling Yang Xinyue Wang Ciqiu Yang Jie Li Jie Li Dongyang Yang Dongyang Yang |
| author_facet | Qinkai Zhang Qinkai Zhang Qinkai Zhang Ziyu Gao Ru Qiu Jizhao Cao Chunxiao Zhang Wei Qin Wei Qin Meiling Yang Meiling Yang Xinyue Wang Ciqiu Yang Jie Li Jie Li Dongyang Yang Dongyang Yang |
| author_sort | Qinkai Zhang |
| collection | DOAJ |
| description | Small cell carcinoma of the esophagus (SCCE) is a rare and aggressively progressing malignancy that presents considerable clinical challenges.Although chemotherapy can effectively manage symptoms during the earlystages of SCCE, its long-term effectiveness is notably limited, with theunderlying mechanisms remaining largely undefined. In this study, weemployed single-cell RNA sequencing (scRNA-seq) to analyze SCCE samplesfrom a single patient both before and after chemotherapy treatment. Our analysisrevealed significant cellular plasticity and alterations in the tumormicroenvironment’s cellular composition. Notably, we observed an increase intumor cell diversity coupled with reductions in T cells, B cells, and myeloid-likecells. The pre-treatment samples predominantly featured carcinoma cells in amiddle transitional state, while post-treatment samples exhibited an expandedpresence of cells in terminal, initial-to-terminal (IniTerm), and universally alteredstates. Further analysis highlighted dynamic interactions between tumor cells andimmune cells, with significant changes detected in key signaling pathways, suchas TIGIT-PVR and MDK-SDC4. This study elucidates the complex dynamics of cellplasticity in SCCE following chemotherapy, providing new insights and identifyingpotential therapeutic targets to enhance treatment efficacy. |
| format | Article |
| id | doaj-art-18ef891e0070499bbb267007af8a6017 |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-18ef891e0070499bbb267007af8a60172025-01-09T06:11:02ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-01-011510.3389/fgene.2024.14777051477705Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatmentQinkai Zhang0Qinkai Zhang1Qinkai Zhang2Ziyu Gao3Ru Qiu4Jizhao Cao5Chunxiao Zhang6Wei Qin7Wei Qin8Meiling Yang9Meiling Yang10Xinyue Wang11Ciqiu Yang12Jie Li13Jie Li14Dongyang Yang15Dongyang Yang16Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, Guangdong, ChinaDepartment of Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaSchool of Laboratory Medicine, Guangzhou Health Science College, Guangzhou, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaMedical College, Jiaying University, Meizhou, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, ChinaMedical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, ChinaMedical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, Guangzhou Women and Children’s Medical Center, Guangzhou, Guangdong, ChinaDepartment of Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, ChinaMedical Research Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaMedical Oncology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaSmall cell carcinoma of the esophagus (SCCE) is a rare and aggressively progressing malignancy that presents considerable clinical challenges.Although chemotherapy can effectively manage symptoms during the earlystages of SCCE, its long-term effectiveness is notably limited, with theunderlying mechanisms remaining largely undefined. In this study, weemployed single-cell RNA sequencing (scRNA-seq) to analyze SCCE samplesfrom a single patient both before and after chemotherapy treatment. Our analysisrevealed significant cellular plasticity and alterations in the tumormicroenvironment’s cellular composition. Notably, we observed an increase intumor cell diversity coupled with reductions in T cells, B cells, and myeloid-likecells. The pre-treatment samples predominantly featured carcinoma cells in amiddle transitional state, while post-treatment samples exhibited an expandedpresence of cells in terminal, initial-to-terminal (IniTerm), and universally alteredstates. Further analysis highlighted dynamic interactions between tumor cells andimmune cells, with significant changes detected in key signaling pathways, suchas TIGIT-PVR and MDK-SDC4. This study elucidates the complex dynamics of cellplasticity in SCCE following chemotherapy, providing new insights and identifyingpotential therapeutic targets to enhance treatment efficacy.https://www.frontiersin.org/articles/10.3389/fgene.2024.1477705/fullsmall cell carcinoma of the esophagussingle-cell RNA sequencingchemotherapytumor microenvironmentcell plasticity |
| spellingShingle | Qinkai Zhang Qinkai Zhang Qinkai Zhang Ziyu Gao Ru Qiu Jizhao Cao Chunxiao Zhang Wei Qin Wei Qin Meiling Yang Meiling Yang Xinyue Wang Ciqiu Yang Jie Li Jie Li Dongyang Yang Dongyang Yang Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment Frontiers in Genetics small cell carcinoma of the esophagus single-cell RNA sequencing chemotherapy tumor microenvironment cell plasticity |
| title | Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| title_full | Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| title_fullStr | Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| title_full_unstemmed | Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| title_short | Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| title_sort | single cell rna sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment |
| topic | small cell carcinoma of the esophagus single-cell RNA sequencing chemotherapy tumor microenvironment cell plasticity |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1477705/full |
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