Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity
Summary: Pseudomonas syringae deploys a type III secretion system (T3SS) to deliver effector proteins to facilitate infection of plant cells; however, little is known about the direct interactions between T3SS components and plants. Here, we show that the specialized lytic transglycosylase (SLT) dom...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724014967 |
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| author | Jun-Zhou Li Yi-Lin Gu Wei Zhang Shen Cong Ruo-Na Wang Yi-Nan Ma Ya Jin Hai-Lei Wei |
| author_facet | Jun-Zhou Li Yi-Lin Gu Wei Zhang Shen Cong Ruo-Na Wang Yi-Nan Ma Ya Jin Hai-Lei Wei |
| author_sort | Jun-Zhou Li |
| collection | DOAJ |
| description | Summary: Pseudomonas syringae deploys a type III secretion system (T3SS) to deliver effector proteins to facilitate infection of plant cells; however, little is known about the direct interactions between T3SS components and plants. Here, we show that the specialized lytic transglycosylase (SLT) domain of P. syringae pv. tomato (Pst) DC3000 T3SS component HrpH is necessary for effector translocation. HrpH and its SLT domain induce host cell death and suppress pattern-triggered immunity (PTI). Transgenic hrpH-Arabidopsis plants exhibit decreased PTI responses and enhanced susceptibility to Pst DC3000ΔhrcQ-U. HrpH suppresses salicylic acid (SA) signaling and interacts with the E3 ubiquitin ligase ATL2 via its SLT domain, independent of its catalytic glutamate. ATL2 silencing indicates that ATL2 is required for basal resistance to bacterial infection, HrpH-triggered cell death, and suppressing MAPK and SA signaling. Our findings highlight that beyond serving as a lytic transglycosylase for effector delivery, HrpH targets an E3 ligase to modulate plant immunity. |
| format | Article |
| id | doaj-art-18ca851c64dc47209071e8152aa71e38 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-18ca851c64dc47209071e8152aa71e382025-01-04T04:56:16ZengElsevierCell Reports2211-12472025-01-01441115145Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunityJun-Zhou Li0Yi-Lin Gu1Wei Zhang2Shen Cong3Ruo-Na Wang4Yi-Nan Ma5Ya Jin6Hai-Lei Wei7State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaPlant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA; Corresponding authorState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authorSummary: Pseudomonas syringae deploys a type III secretion system (T3SS) to deliver effector proteins to facilitate infection of plant cells; however, little is known about the direct interactions between T3SS components and plants. Here, we show that the specialized lytic transglycosylase (SLT) domain of P. syringae pv. tomato (Pst) DC3000 T3SS component HrpH is necessary for effector translocation. HrpH and its SLT domain induce host cell death and suppress pattern-triggered immunity (PTI). Transgenic hrpH-Arabidopsis plants exhibit decreased PTI responses and enhanced susceptibility to Pst DC3000ΔhrcQ-U. HrpH suppresses salicylic acid (SA) signaling and interacts with the E3 ubiquitin ligase ATL2 via its SLT domain, independent of its catalytic glutamate. ATL2 silencing indicates that ATL2 is required for basal resistance to bacterial infection, HrpH-triggered cell death, and suppressing MAPK and SA signaling. Our findings highlight that beyond serving as a lytic transglycosylase for effector delivery, HrpH targets an E3 ligase to modulate plant immunity.http://www.sciencedirect.com/science/article/pii/S2211124724014967CP: MicrobiologyCP: Plants |
| spellingShingle | Jun-Zhou Li Yi-Lin Gu Wei Zhang Shen Cong Ruo-Na Wang Yi-Nan Ma Ya Jin Hai-Lei Wei Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity Cell Reports CP: Microbiology CP: Plants |
| title | Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity |
| title_full | Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity |
| title_fullStr | Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity |
| title_full_unstemmed | Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity |
| title_short | Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity |
| title_sort | pseudomonas syringae lytic transglycosylase hrph interacts with host ubiquitin ligase atl2 to modulate plant immunity |
| topic | CP: Microbiology CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724014967 |
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