Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses
Abstract We engineered a microfluidic platform to study the effects of bioactive glass nanoparticles (BGNs) on cell viability under static culture. We incorporated different concentrations of BGNs (1%, 2%, and 3% w/v) in collagen hydrogel (with a concentration of 3.0 mg/mL). The microfluidic chip’s...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-84346-8 |
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| author | Faezeh Ghobadi Maryam Saadatmand Sara Simorgh Peiman Brouki Milan |
| author_facet | Faezeh Ghobadi Maryam Saadatmand Sara Simorgh Peiman Brouki Milan |
| author_sort | Faezeh Ghobadi |
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| description | Abstract We engineered a microfluidic platform to study the effects of bioactive glass nanoparticles (BGNs) on cell viability under static culture. We incorporated different concentrations of BGNs (1%, 2%, and 3% w/v) in collagen hydrogel (with a concentration of 3.0 mg/mL). The microfluidic chip’s dimensions were optimized through fluid flow and mass transfer simulations. Collagen type I extracted from rat tail tendons was used as the main material, and BGNs synthesized by the sol–gel method were used to enhance the mechanical properties of the hydrogel. The extracted collagen was characterized using FTIR and SDS-PAGE, and BGNs were analyzed using XRD, FTIR, DLS, and FE-SEM/EDX. The structure of the collagen-BGNs hydrogels was examined using SEM, and their mechanical properties were determined using rheological analysis. The cytotoxicity of BGNs was assessed using the MTT assay, and the viability of fibroblast (L929) cells encapsulated in the collagen-BGNs hydrogel inside the microfluidic device was assessed using a live/dead assay. Based on all these test results, the L929 cells showed high cell viability in vitro and promising microenvironment mimicry in a microfluidic device. Collagen3-BGNs3 (Collagen 3 mg/mL + BGNs 3% (w/v)) was chosen as the most suitable sample for further research on a microfluidic platform. |
| format | Article |
| id | doaj-art-18bb8ddcc0f44a968ab96c3972e57d7f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-18bb8ddcc0f44a968ab96c3972e57d7f2025-01-05T12:16:59ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-84346-8Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glassesFaezeh Ghobadi0Maryam Saadatmand1Sara Simorgh2Peiman Brouki Milan3Department of Chemical and Petroleum Engineering, Sharif University of TechnologyDepartment of Chemical and Petroleum Engineering, Sharif University of TechnologyCellular and Molecular Research Center, Iran University of Medical SciencesCellular and Molecular Research Center, Iran University of Medical SciencesAbstract We engineered a microfluidic platform to study the effects of bioactive glass nanoparticles (BGNs) on cell viability under static culture. We incorporated different concentrations of BGNs (1%, 2%, and 3% w/v) in collagen hydrogel (with a concentration of 3.0 mg/mL). The microfluidic chip’s dimensions were optimized through fluid flow and mass transfer simulations. Collagen type I extracted from rat tail tendons was used as the main material, and BGNs synthesized by the sol–gel method were used to enhance the mechanical properties of the hydrogel. The extracted collagen was characterized using FTIR and SDS-PAGE, and BGNs were analyzed using XRD, FTIR, DLS, and FE-SEM/EDX. The structure of the collagen-BGNs hydrogels was examined using SEM, and their mechanical properties were determined using rheological analysis. The cytotoxicity of BGNs was assessed using the MTT assay, and the viability of fibroblast (L929) cells encapsulated in the collagen-BGNs hydrogel inside the microfluidic device was assessed using a live/dead assay. Based on all these test results, the L929 cells showed high cell viability in vitro and promising microenvironment mimicry in a microfluidic device. Collagen3-BGNs3 (Collagen 3 mg/mL + BGNs 3% (w/v)) was chosen as the most suitable sample for further research on a microfluidic platform.https://doi.org/10.1038/s41598-024-84346-8Microfluidic system3D cell cultureCollagen hydrogelBioactive glass nanoparticle |
| spellingShingle | Faezeh Ghobadi Maryam Saadatmand Sara Simorgh Peiman Brouki Milan Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses Scientific Reports Microfluidic system 3D cell culture Collagen hydrogel Bioactive glass nanoparticle |
| title | Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| title_full | Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| title_fullStr | Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| title_full_unstemmed | Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| title_short | Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| title_sort | microfluidic 3d cell culture potential application of collagen hydrogels with an optimal dose of bioactive glasses |
| topic | Microfluidic system 3D cell culture Collagen hydrogel Bioactive glass nanoparticle |
| url | https://doi.org/10.1038/s41598-024-84346-8 |
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