Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis.
<h4>Background</h4>The SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRN...
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Public Library of Science (PLoS)
2024-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0313042 |
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| author | Marwa A Ali Olfat G Shaker Eman M Ezzat El Shaimaa Gomaa Ali Mahmoud I Aboelor Mona I Ahmed Essam A Hassan Doaa Y Ali Heba Mostafa Ahmed Abeer A Khalefa Rasha A Hussein Ahmed Fathy Elkhateeb Esam Ali Mohamed |
| author_facet | Marwa A Ali Olfat G Shaker Eman M Ezzat El Shaimaa Gomaa Ali Mahmoud I Aboelor Mona I Ahmed Essam A Hassan Doaa Y Ali Heba Mostafa Ahmed Abeer A Khalefa Rasha A Hussein Ahmed Fathy Elkhateeb Esam Ali Mohamed |
| author_sort | Marwa A Ali |
| collection | DOAJ |
| description | <h4>Background</h4>The SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies for the disease.<h4>Aim</h4>Our study aimed to measure NEAT-1, miR-374b-5p, and IL6 in the serum of COVID-19 patients, demonstrating the correlation between target genes to explore the possible relationship between them. Also, the association between target genes and patients' clinical findings and radiological severity indices will be explored.<h4>Patients and methods</h4>The current study included 48 COVID-19-infected individuals and 40 controls. Quantitative real-time PCR (qPCR) was performed to detect lncRNA NEAT-1 and miRNA374b-5p fold change (FC) in the participants' sera. Enzyme-Linked Immune Sorbent Assay (ELISA) is used to detect IL6.<h4>Results</h4>Our results showed statistical significance with lower levels of (NEAT-1) [ median (range) = 0.08 (0.001-0.602)], and (miR374b-5p) [ median (range) = 0.14 (.01-7.16)] while higher IL-6 levels [ median (range) = 41.3 (7.2-654) pg/ml] when compared to controls with p-value <0.001. Serum level of NEAT-1 correlates negatively with IL-6 level (r = -.317, P = .008). ROC curve analysis revealed that sensitivity and specificity tests for NEAT-1 and IL-6 levels in the diagnosis of cases illustrated a sensitivity of (100% and 97.9%) and a specificity of (85% and 100%) at cut-off values (0.985 and 12.55), respectively. In comparison, miR374b-5p showed sensitivity and specificity of around 85% in distinguishing COVID-19 patients from controls. No significant association was detected between target genes and radiological severity indices.<h4>Conclusions</h4>Our study is the first to detect decreased NEAT-1 and miR374b-5p expression in COVID-19 patients' serum. There was also an increase in IL6 levels. There is a negative correlation between NEAT-1 and IL6 in COVID-19 patients. |
| format | Article |
| id | doaj-art-186998ee3af94b70946b104ab2446640 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-186998ee3af94b70946b104ab24466402025-01-08T05:32:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031304210.1371/journal.pone.0313042Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis.Marwa A AliOlfat G ShakerEman M EzzatEl Shaimaa Gomaa AliMahmoud I AboelorMona I AhmedEssam A HassanDoaa Y AliHeba Mostafa AhmedAbeer A KhalefaRasha A HusseinAhmed Fathy ElkhateebEsam Ali Mohamed<h4>Background</h4>The SARS-CoV-2 virus's frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is a need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies for the disease.<h4>Aim</h4>Our study aimed to measure NEAT-1, miR-374b-5p, and IL6 in the serum of COVID-19 patients, demonstrating the correlation between target genes to explore the possible relationship between them. Also, the association between target genes and patients' clinical findings and radiological severity indices will be explored.<h4>Patients and methods</h4>The current study included 48 COVID-19-infected individuals and 40 controls. Quantitative real-time PCR (qPCR) was performed to detect lncRNA NEAT-1 and miRNA374b-5p fold change (FC) in the participants' sera. Enzyme-Linked Immune Sorbent Assay (ELISA) is used to detect IL6.<h4>Results</h4>Our results showed statistical significance with lower levels of (NEAT-1) [ median (range) = 0.08 (0.001-0.602)], and (miR374b-5p) [ median (range) = 0.14 (.01-7.16)] while higher IL-6 levels [ median (range) = 41.3 (7.2-654) pg/ml] when compared to controls with p-value <0.001. Serum level of NEAT-1 correlates negatively with IL-6 level (r = -.317, P = .008). ROC curve analysis revealed that sensitivity and specificity tests for NEAT-1 and IL-6 levels in the diagnosis of cases illustrated a sensitivity of (100% and 97.9%) and a specificity of (85% and 100%) at cut-off values (0.985 and 12.55), respectively. In comparison, miR374b-5p showed sensitivity and specificity of around 85% in distinguishing COVID-19 patients from controls. No significant association was detected between target genes and radiological severity indices.<h4>Conclusions</h4>Our study is the first to detect decreased NEAT-1 and miR374b-5p expression in COVID-19 patients' serum. There was also an increase in IL6 levels. There is a negative correlation between NEAT-1 and IL6 in COVID-19 patients.https://doi.org/10.1371/journal.pone.0313042 |
| spellingShingle | Marwa A Ali Olfat G Shaker Eman M Ezzat El Shaimaa Gomaa Ali Mahmoud I Aboelor Mona I Ahmed Essam A Hassan Doaa Y Ali Heba Mostafa Ahmed Abeer A Khalefa Rasha A Hussein Ahmed Fathy Elkhateeb Esam Ali Mohamed Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. PLoS ONE |
| title | Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. |
| title_full | Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. |
| title_fullStr | Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. |
| title_full_unstemmed | Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. |
| title_short | Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients' diagnosis and prognosis. |
| title_sort | peripheral lncrna neat 1 mir374b 5p and il6 panel to guide in covid 19 patients diagnosis and prognosis |
| url | https://doi.org/10.1371/journal.pone.0313042 |
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