OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY
Backround: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesisAim: The aim of this study is to invest...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-04-01
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| Series: | Selcuk Dental Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/1029627 |
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| Summary: | Backround: Diabetes
mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone
have several actions such as
antimicrobial ,immunostimulating, antihypoxic effects, and activates
antioxidant enzymes and angiogenesisAim:
The aim of this study is to investigate the
effect of gaseous ozone on bone healing in diabetic rat calvarial defects
treated with autogenous bone graft, morphometrically and immunohistochemically.Material and Methods:
Diabetes was induced and critical size defects were created on rats. Study
groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group,
3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone
application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation
day and the following 2 weeks daily (140ppm @
2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic
protein-2 protein expressions were evaluated.Results: Control and Control+Ozone groups had no osteoclast and
residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher
than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area
than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity
compared to the other groups. Osteocalcin immune positivity in AG groups was
higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozone application
decreased osteoclast number and increased osteoblast number and bone
regeneration, especially, in early stages of bone regeneration in diabetic
rats.
KEYWORDS: autograft, bone regeneration, calvarial defect, experimental
diabetes mellitus, ozone |
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| ISSN: | 2148-7529 |