Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties

Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties

We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its antigen specificity by Western blotting and competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues and natural viral antige...

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Main Authors: G. I. Alatortseva, A. V. Sidorov, L. N. Nesterenko, L. N. Luhverchik, I. I. Amiantova, V. V. Docenko, Phong Pham Huy, A. V. Milovanova, O. B. Vylivannaya, A. C. Kazanova, V. V. Lavrov, V. V. Zverev
Format: Article
Language:Russian
Published: Numikom LLC 2016-02-01
Series:Эпидемиология и вакцинопрофилактика
Subjects:
вирус varicella zoster
orf68
гликопротеин е
рекомбинантный антиген
иммуноферментный анализ
varicella zoster virus, vzv
open reading frame 68, orf 68
glycoprotein g
recombinant antigen
enzyme immunoassay
Online Access:https://www.epidemvac.ru/jour/article/view/131
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