Protocol for the generation and immunostaining of thick murine lung tissue slices
Summary: Precision-cut lung slices (PCLSs) largely preserve the native structural and cellular complexity of the lung, enabling detailed spatial analysis of resident cell populations. Here, we present a protocol for generating PCLSs from mouse lungs for subsequent immunofluorescent staining to detec...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500440X |
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